基于斑马鱼胚胎毒性与非靶化学分析的典型工业园区废水处理效率研究

Treatment efficiency of typical industrial wastewater based on zebrafish embryo toxicity and non-target analysis

工业园区废水中污染物种类复杂、毒性大、去除难,在处理出水水质达标的情况下仍可能存在生态健康风险,因此,有效评估园区废水的处理效率具有重要意义.采用斑马鱼胚胎毒性实验和基于液相色谱-质谱的非靶标污染物筛查方法分析了两家典型工业园区污水处理厂的处理效率.研究结果表明,生物毒性检测和非靶标化学分析可从不同侧面解释废水中毒害污染物在水处理过程中的沿程变化情况,两者的研究结果相互支撑.两类分析方法都发现,生化处理工艺无法有效去除工业废水中斑马鱼胚胎发育毒性物质.高级氧化技术、吸附技术和膜生物反应器技术等可以进一步有效削减污染物丰度及毒性,市政污水的混入提升了工业废水中毒害污染物的去除率及生物毒性的削减率.本研究结果证实了斑马鱼胚胎毒性与非靶化学分析可用于确定工业废水处理的效率,对工业废水处理过程的设计和调控具有指导意义.

Organic pollutants of wastewater in industrial parks are generally complex,highly toxic and difficult to remove,which may still be potential ecotoxic effects after treatment. Therefore,it is of great significant to evaluate the treatment efficiency of wastewater in the industrial parks. In this study,zebrafish embryo test and non-target contaminant screening method based on liquid chromatography-mass spectrometry(LC-MS)were used to analyze the treatment efficiency of two typical industrial park wastewater treatment plants. The combination of biological endpoints and chemical analyses contributed to explaining the changes of toxic pollutants in wastewater along the treatment process. results demonstrated that the biochemical treatment process could not effectively remove zebrafish embryotoxic substances in industrial wastewater. The advanced oxidation processes,adsorption process,and membrane bio-reacter(MBR)based process further eliminated pollutants and their toxicities. The combination of municipal and industrial wastewater enhanced the removal of toxic pollutants in industrial wastewater and reduced biological toxicity. Overall,it is feasible to combine zebrafish embryotoxicity and non-target chemical screening to determine the efficiency of industrial wastewater treatment,which is helpful in guiding the design and regulation of the industrial wastewater treatment process.