离子色谱法测定小儿止咳糖浆中氯化铵的含量

Determination of Ammonium Chloride in Xiao’er Zhike Syrup by Ion Chromatography

目的建立离子色谱法测定小儿止咳糖浆中氯化铵含量的方法。方法离子色谱法采用IonPac^(TM) AS11-HC阴离子交换色谱柱(2.0 mm×250 mm)与IonPac^(TM) AG11-HC保护柱(2.0 mm×50 mm),检测器为抑制电导检测器,电导检测池温度为35℃,柱温为30℃,进样量为25μL,以10 mmol·L^(-1)氢氧化钾溶液为流动相,流速为0.5 mL·min^(-1)。结果氯离子浓度为10~400μg·mL^(-1)时,峰面积与其浓度呈良好的线性关系(r=1.0000,n=6);平均回收率为100.57%(RSD=0.41%,n=6)。结论该方法专属性强、准确度高,可用于小儿止咳糖浆中氯化铵的含量测定。

OBJECTIVE To establish an ion chromatography method for determination of ammonium chloride in Xiao’er Zhike syrup.METHODS The chromatographic separation was performed on an IonPac^(TM) AS11-HC anion exchange column(2.0 mm×250 mm)and IonPac^(TM) AG11-HC guard column(2.0 mm×50 mm),using suppressed conductivity detector,the temperature of the detection tank and the column were set at 35℃ and 30℃ respectively,the injection volume was 25μL,an isocratic eluent of 10 mmol·L^(-1) KOH was chosen as mobile phase and the flow rate was 0.5 mL·min^(-1).RESULTS A good linear relationship between the peak area and its concentration was observed within the range of 10−400μg·mL^(-1)(r=1.0000,n=6);The average recovery was 100.57%(RSD=0.41%,n=6).CONCLUSION The method is specific and accurate,and can be used for determination of ammonium chloride in Xiao’er Zhike syrup.