抗骨质疏松药物对双足鼠脊柱侧弯模型的影响

Effect of two anti-osteoporosis drugs on scoliosis progression in bipedal mice

[目的]探讨阿仑膦酸钠(alendronate, AL)和特立帕肽(teriparatide, TPTD)对双足鼠脊柱侧弯进展的作用。[方法]将45只C57BL/6J雌性4周龄小鼠行双前肢和尾离断,建立双足鼠脊柱侧弯模型,随机分为3组,每组15只。对照组、AL组和TPTD组,分别给予生理盐水、AL和TPTD,连续12周。行影像和骨密度(bone mineral density, BMD)检测。[结果]随时间推移,三组动物Cobb角均显著增加(P<0.05)。术后8周和12周,脊柱侧弯Cobb角由大至小依次为:对照组>AL组>TPTD组,总体差异有统计学意义(P<0.05);两两比较,对照组的Cobb角显著大于AL组和TPTD组(P<0.05),而AL组与TPTD组间差异无统计学意义(P>0.05)。随时间推移,三组动物BMD均显著增加(P<0.05)。术后4、8和12周BMD值由低至高均为:对照组<AL组<TPTD组,总体差异有统计学意义(P<0.05)。两两比较方面,对照组的BMD与AL组和TPTD组的差异均有统计学意义(P<0.05),而AL组与TPTD组间的BMD差异均无统计学(P>0.05)。[结论]在双足鼠脊柱侧弯模型早期给予AL和TPTD可有效减小鼠脊柱侧弯Cobb角,同时增加生长期小鼠脊柱BMD值。

[Objective]To observe the effects of alendronate(AL)and teriparatide(TPTD)on scoliosis progression in bipedal mice.[Methods]Forty-five 4-week-old female C57BL/6J mice received bilateral forelimb amputation to establish a scoliosis model of bipedal mice.The animals were randomly divided into 3 groups with 15 mice in each group,including the control group,AL group and TPTD group,which were given normal saline,AL and TPTD respectively for 12 weeks.The imaging check and bone mineral density(BMD)assay were conducted.[Results]The Cobb angle increased significantly over time in all the 3 groups(P<0.05).At 8 and 12 weeks after amputation,the Cobb angle of scoliosis was ranked in descending order of control group>AL group>TPTD group with statistically significant overall difference(P>0.05),which in the control group was significantly higher than that of AL group and TPTD group(P<0.05),whereas there was no significant difference between AL group and TPTD group(P>0.05).In addition,the BMD significantly increased over time in all three groups(P<0.05),which ranked from low to high at 4,8 and 12 weeks after amputation were as follows:control group<AL group<TPTD group,with statistically significant overall difference(P<0.05).In term of pairwise comparison,the control group had significantly lower BMD than the AL and TPTD groups(P<0.05),whereas there was no significant difference in BMD between AL group and TPTD group(P>0.05).[Conclusion]Early administration of AL and TPTD in bipedal mice scoliosis model can effectively reduce Cobb angle and increase spinal BMD in growing mice.