羟基磷灰石-氧化石墨烯复合微球的制备及性能

Preparation and performance of hydroxyapatitegraphene oxide composite microspheres

载药微球目前在生物医用材料方面得到广泛研究,但存在载药量不高和突释等问题,为解决此类问题,利用羟基磷灰石(hydroxyapatite,HA)和氧化石墨烯(graphene oxide,GO)制备羟基磷灰石-氧化石墨烯(HA-GO)复合微球.首先采用硬模板法合成球状碳酸钙-氧化石墨烯(CaCO_(3)-GO)复合材料,然后通过水热法以离子交换方式成功制备了球状中空HA-GO复合微球,研究不同合成条件对HA-GO复合材料的影响.通过X射线粉末衍射、傅里叶红外光谱、拉曼红外光谱、场发射扫描电子显微镜和紫外可见分光光度计等测试方法对所制备样品进行分析和表征,并以姜黄素作为载药模型对复合微球载药性能进行测试,通过包封率及载药量两个指标对复合微球的载药性能进行评估,同时测试微球样品材料的细胞毒性.研究表明:体系反应物的浓度及水热反应时间对复合微球的成型效果影响较大,在初始反应物浓度为0.3 mol/L、水热反应时间为6 h时,可制得形貌良好的中空HA-GO复合微球,所制的复合微球粒径为5.1~7.7μm,孔径约为40 nm.研究还发现,球状结构能提高药物的负载量,复合微球的药物包封效率为(20.90±0.31)%,载药量为(2.95±0.19)%.由此表明,该方法制备的HA-GO复合微球有良好的医用价值.

Drug-loaded microspheres have been widely studied and applied in biomedical materials,but there are still some open problems such as low drug loading and sudden release.In order to solve such problems,hydroxyapatite-graphene oxide(HA-GO)composite microspheres were prepared by using hydroxyapatite(HA)and graphene oxide(GO).Firstly,spherical calcium carbonate-graphene oxide(CaCO_(3)-GO)composites were synthesized by hard template method.Then,spherical hollow HA-GO composite microspheres were successfully prepared by a method of hydrothermal-assisted ion exchange.The effect of different synthesis conditions on the prepared HA-GO composites was studied.Through a series of measurements,such as X-ray powder diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),Raman infrared spectroscopy,field emission scanning electron microscope(FESEM),ultraviolet visible spectrophotometer(UV-VIS),etc.,the prepared samples were analyzed and characterized.The drug-loading performance of the microspheres was tested by using curcumin as the drug-loading model,and the drug-loading performance was evaluated by two indicators of encapsulation efficiency and drug-loading capacity.At the same time,the cytotoxicity test of the microsphere sample materials was also performed.The research results show that the reactant concentration of the system and the hydrothermal reaction time greatly affect the forming effect of the composite microspheres.When the initial reactant concentration is 0.3 mol/L and the hydrothermal reaction time is 6 h,hollow HA-GO composite microspheres with good morphology can be prepared,with a particle size from 5.1μm to 7.7μm and a pore size of about 40 nm.At the same time,it was found that the spherical structure can improve the drug loading,the drug encapsulation efficiency was(20.90±0.31)%,and the drug loading was(2.95±0.19)%.This shows that HA-GO composite microspheres have good medical application value.