基于JNK/AMPK-mTOR调控Bcl-XL对急性髓系白血病细胞化疗敏感度的干预作用

Intervention of JNK/AMPK-mTOR regulation of Bcl-XL on the chemo-therapy sensitivity of acute myeloid leukemia cells

目的探究基于JNK/AMPK-mTOR调控Bcl-XL对急性髓系白血病细胞化疗敏感度的干预作用。方法选取2020年1月~2021年1月在台州市中心医院进行的实验,白血病HL-60细胞株经过细胞培养、转染后,分为下调Bcl-XL组、急性髓系白血病组和阴性对照组。免疫细胞化学染色检测Bcl-XL,免疫印迹检测JNK/AMPK-mTOR相关蛋白的表达。结果下调Bcl-XL组Bcl-XL表达低于急性髓系白血病组和阴性对照组(均P<0.05)。在24、48、72 h,下调Bcl-XL组细胞吸光密度值、细胞增殖数均低于急性髓系白血病组和阴性对照组,细胞凋亡数高于急性髓系白血病组和阴性对照组(均P<0.05)。下调Bcl-XL组mTOR、P70S6K、P-JNK、AMPK、IC50值低于其他急性髓系白血病组和阴性对照组(均P<0.05)。结论下调Bcl-XL可影响JNK/AMPK-mTOR信号通路的活性,参与急性髓系白血病细胞增殖、凋亡的过程,在急性髓系白血病中起作用。

Objective To explore the intervention effect of JNK/AMPK-mTOR regulation of Bcl-XL on chemotherapy sensitivity in acute myeloid leukemia cells.Methods Based on experiments conducted in Taizhou Central Hospital from January 2020 to January 2021,leukemia HL-60 cell lines after cell culture and transfection were divided into regulated Bcl-XL,acute myeloid leukemia and negative control groups.The expression of JNK/AMPK-mTOR-associated proteins was determined by Bcl-XL,immunoblotting by immunocytochemical staining.Results Bcl-XL expression was lower in the downregulated Bcl-XL group than in the acute myeloid leukemia and negative control groups(all P<0.05).Cell absorption density values and cell proliferation in the Bcl-XL group at 24 h,48 h,72 h,were lower than in the acute myeloid leukemia and negative control groups and apoptosis was higher than in the acute myeloid leukemia and negative control groups(all P<0.05).The mTOR,P70S6K,P-JNK,AMPK,IC50 in the Bcl-XL group was lower than in the acute myeloid leukemia and negative control groups(all P<0.05).Conclusion Downregulation of Bcl-XL can affect the activity of the JNK/AMPK-mTOR signaling pathway,participate in the process of acute myeloid leukemia cell proliferation,apoptosis,and play a role in acute myeloid leukemia.