血清淀粉样蛋白A在儿童轮状病毒肠炎诊断中的应用

Application of Serum Amyloid A in the Diagnosis of Rotavirus Enteritis in Children

目的探讨血清淀粉样蛋白A(SAA)在儿童轮状病毒肠炎诊断中的应用。方法选取120例胶体金法检测粪便后轮状病毒呈阳性的肠炎患儿进行回顾性分析,将其分为观察组,另选取120例由非感染原因导致的功能性腹泻患儿作为对照组,对比两组患儿血清SAA表达水平与阳性率,分析不同脱水程度轮状病毒肠炎患儿SAA表达水平与阳性率,根据血清SAA得出对应特异度和敏感度,纵坐标为敏感度,横坐标为特异度,进行受试者工作(ROC)曲线绘制,分析SAA对儿童轮状病毒肠炎的诊断价值。结果观察组与对照组患儿SAA表达、SAA阳性率对比差异显著,观察组明显高于对照组(P<0.05);不同脱水程度轮状病毒肠炎患儿SAA表达、SAA阳性率对比差异显著,且重度脱水患儿明显高于轻中度脱水和无脱水患儿(P<0.05);血清淀粉样蛋白A的曲线下面积为78.89,最佳诊断界限值为12.52mg/L。结论轮状病毒肠炎患儿血清淀粉样蛋白A表达水平与阳性率相比于功能性腹泻患儿明显升高,且SAA>12.52mg/L为轮状病毒肠炎的诊断临界值。

Objective To explore the application of serum amyloid A(SAA)in the diagnosis of rotavirus enteritis in children.Methods 120 children with enteritis who were positive for fecal rotavirus by colloidal gold method were selected for retrospective analysis.They were divided into observation group and 120 children with functional diarrhea caused by non infectious causes were selected as control group.The expression level and positive rate of serum SAA in the two groups were compared,The expression level and positive rate of SAA in children with rotavirus enteritis with different degrees of dehydration were analyzed.The corresponding specificity and sensitivity were obtained according to serum SAA.The ordinate was the sensitivity and the abscissa was the specificity.The receiver operating(ROC)curve was drawn to analyze the diagnostic value of SAA in children with rotavirus enteritis.Results There were significant differences in SAA expression and SAA positive rates between the observation group and the control group,and the observation group was significantly higher than the control group(P<0.05);the SAA expression and SAA positive rates in children with rotavirus enteritis with different degrees of dehydration were significantly different,And children with severe dehydration were significantly higher than those with mild to moderate dehydration and without dehydration(P<0.05);the area under the curve of serum amyloid A was 78.89,and the best diagnostic limit was 12.52 mg/L.Conclusion The expression level and positive rate of serum amyloid A in children with rotavirus enteritis were significantly higher than those in children with functional diarrhea,and SAA>12.52 mg/L was the critical value for the diagnosis of rotavirus enteritis.