儿童肺炎支原体感染检测应用胶体金法和被动颗粒凝集法的价值比较

Comparison of the Value of Colloidal Gold Method and Passive Particle Agglutination Method in Detecting Mycoplasma Pneumoniae Infection in Children

目的:探讨胶体金法和被动颗粒凝集法应用于儿童肺炎支原体感染检测的临床价值。方法:选取2019年3月-2020年8月于某院就诊112例高度符合儿童MP感染典型症状的患儿作为研究对象,以快速培养鉴定镜检法为金标准,分别应用胶体金法和被动颗粒凝集法进行检测,比较两种检测方法的诊断效能,分析两种检测方法的应用价值。结果:本研究纳入的112例患儿中,快速培养鉴定镜检法共检出阳性患儿76例。胶体金法和被动颗粒凝集法对相同病程的患儿的阳性检测率无明显差异(P>0.05);两种检测方法对病程≥7d患儿的阳性检出率较病程<7d的患儿明显偏高(P<0.05)。两种检测方法对相同年龄段患儿的阳性检出率对比,无显著差异(P>0.05);在不同年龄段患儿中,两种检测手段对7~14岁患儿的阳性检出率明显高于60d~6岁组患儿(P<0.05)。颗粒凝集法的阳性检出率为58.93%(66/112),灵敏度为86.84%,特异度为86.11%,准确度为86.61%;胶体金法的阳性检出率为60.71%(68/112),灵敏度为89.47%,特异度为88.89%,准确度为89.29%;组间对比,胶体金法对儿童肺炎支原体感染的检测效能更佳,但数据差异无统计学意义(P>0.05)。结论:胶体金法于被动颗粒凝集法对儿童肺炎支原体感染检测效能均较好,但胶体金法的操作更为简单便捷、耗时少,便于在基层医院普及应用。

Objective:To explore the clinical value of colloidal gold method and passive particle agglutination method in the detection of mycoplasma pneumoniae infection in children.Methods:A total of 112 cases of patients who highly met the typical symptoms of MP infection in children in a hospital from March 2019 to August 2020 were included as the research subjects.With the rapid culture identification microscopy as the gold standard,colloidal gold method and passive particle agglutination method were used for detection,respectively.The diagnostic efficacy of the two detection methods was compared and the application value of the two detection methods was analyzed.Results:Among the 112 cases included in this study,76 cases were detected as positive by rapid culture identification microscopy.There was no significant difference in the positive detection rate between the colloidal gold method and passive particle agglutination method for children with the same course of disease(P>0.05).The positive detection rate of the two detection methods in children with disease course≥7d was significantly higher than that in children with disease course<7d(P<0.05).There was no significant difference in the positive detection rate between the two detection methods for children with the same age group(P>0.05).For children in different age groups,the positive detection rate of the two detection methods for children aged 7~14 years old was significantly higher than that for children aged 60 d-6 years old(P<0.05).The positive detection rate,sensitivity,specificity and accuracy of particle agglutination method was 58.93%(66/112),86.84%,86.11%and 86.61%,respectively.The positive detection rate,sensitivity,specificity and accuracy of colloidal gold method was 60.71%(68/112),89.47%,88.89%,and 89.29%,respectively.In the inter-group comparison,the colloidal gold method had better detection efficiency for mycoplasma pneumoniae infection in children,but the data difference was not statistically significant(P>0.05).Conclusion:Both colloidal gold method and passive particle agglutination method are effective in the detection of mycoplasma pneumoniae infection in children.However,the operation of colloidal gold method is simpler,more convenient and less time-consuming,which is convenient for promotion and application in primary hospitals.