野生沙杵菇菌株筛选试验

The Wild Coprinus comatus Strain Screening Test

为了挖掘野生沙杵菇新种质,将野外采集的沙杵菇菌丝团与菌蕾采用组织分离法得到71个菌株,利用PDA培养基进行平板培养,观测菌丝颜色、浓密度、均匀度、菌丝长势,并对优选菌株进行了原种培养及出菇试验。结果表明,71个野生沙杵菇菌株在PDA上均能够萌发生长,菌株SHCHG(S)02与SHCHG(L)07菌丝浓白粗壮、均匀浓密、长势强。2个野生菌株经人工栽培,可正常出菇。在熟料栽培下,菌株SHCHG(J)02与SHCHG(L)07产量高,分别达2387和2123 g/筐;生物学转化率分别为39.78%和35.38%,以上2个菌株可用于进一步鉴定、研究、栽培,以培育出产量高、质优的沙杵菇栽培菌种。

In order to explore the new germplasm of wild C.comatus,71 strains were collected from the field by tissue isolation of C.comatus peloton and button.In addition,we conducted plate culture with PDA medium to observe the color of mycelium,density,uniformity,and color of mycelium and carried out original culture and mushroom emergence test on the selected strains.The results showed that 71 wild C.comatus strains were able to germinate and grow on PDA,and the strain SHCHG(S)02 and SHCHG(L)07 mycelium was thick,white,uniform,and dense with strong growth.In addition,2 wild mushroom strains were artificially cultivated and could produce mushrooms typically under clinker cultivation;strains SHCHG(J)02 and SHCHG(L)07 had high yields of 2387 and 2123 g/basket;biological conversion efficiency was 39.78%and 35.38%,respectively.Thus,the above two strains can be used to identify further,research,and cultivate to produce high-yielding and high-quality cultivated strains of Comatus.