联合法诱导内脏高敏感模型的比较研究

Visceral hypersensitivity model of irritable bowel syndrome established by combined methods:a comparative study

目的观察母婴分离联合机械刺激法和药物联合慢性应激法建立的肠易激综合征内脏高敏感性模型的效应,并探讨上述两种方法的优劣。方法将SD幼鼠、成年鼠随机分为母婴分离联合机械刺激组(CRD组)和空白对照组(CRD对照组)、药物联合慢性应激组(CUMS组)与空白对照组(CUMS对照组),模型建立后,观察大鼠一般情况与大便情况,采用腹壁撤退反射(AWR)与内脏痛阈值评价大鼠内脏敏感性,十字迷宫实验与旷场实验评价大鼠焦虑抑郁状态,在上述评价后,取大鼠远端结肠进行病理观察。结果与对应对照组相比,两个模型组大鼠AWR评分增高、内脏痛阈值降低、粪便含水量增高。其中,药物联合CUMS组的IBS内脏高敏感大鼠的十字迷宫开臂停留时间缩短,旷场实验活动总距离与中心停留时间减少。结论两种造模方式诱导的模型均出现IBS内脏高敏感性和粪便性状改变,可较好地模拟IBS临床症状,且更趋向于腹泻型IBS;药物联合CUMS能更好地复制IBS的情绪功能障碍。

Objective To observe the effects of a visceral hypersensitivity model in rats with irritable bowel syndrome established by neonatal maternal separation combined with colorectal distension and drugs with chronic stress,and to discuss their features.Methods Two-day-old and two-month-old Sprague-Dawley rats were randomly divided into CRD,control,and CUMS groups.After establishing the model,the general conditions,stools,visceral sensitivity,and anxiety-depression-like behavioral changes were evaluated.Colonic tissue was obtained for pathological examination.Result Both model groups had a higher AWR score,lower visceral pain threshold,and changed fecal characteristics and water content.The CUMS group had a shorter open arm residence time of the elevated plus maze test and shorter total distance and central area residence time of the open field test.Conclusions Both models showed visceral hypersensitivity and fecal changes,which agreed with the clinical symptoms of IBS,and are more inclined to the diarrhea type.Additionally,the CUMS group model had emotional changes.