黑翅土白蚁工蚁和兵蚁转录组测序比较及表达分析

Comparative Transcriptome Sequencing and Expression Analysis of Worker and Soldier Termites of Odontotermes Formosanus

基于Illumina高通量测序RNA-seq技术对黑翅土白蚁Odontotermes formosanus(Shiraki,1909)的工蚁和兵蚁进行了转录组测序分析,了解不同品级的转录组特征,丰富白蚁转录组数据信息,为探明黑翅土白蚁品级分化的分子机制打下基础。经过测序获得质量值高于20的碱基比例(Q20)均不低于98%的数据,所有reads组装成65604个Unigenes,平均长度337.78 bp;将所得序列注释到NR、NT、KOG、GO和KEGG等数据库进行比对,所得Unigenes均被注释。兵蚁与工蚁差异基因的上调基因有与能量代谢及蛋白质翻译有关,下调相关基因有与能量运输相关,差异基因丰富,有助于黑翅土白蚁品级分化相关基因挖掘。本研究扩充了黑翅土白蚁的基因资源,为科学研究白蚁种群的基因构造、遗传社会性、获取有效分子标记、综合防控等提供珍贵的生物资源。

Based on Illumina high-throughput sequencing RNA-seq technology,the transcriptome of worker and soldier termites of Odontotermes formosanus(Shiraki,1909)was sequenced to understand the transcriptome characteristics of different grades,to enrich the information of termite transcriptome data,and to lay the foundation for exploring the molecular mechanism of grade differentiation in the black-winged soil termite.After sequenced,we obtained data that the proportion of bases with quality values higher than 20(Q20)was not less than 98%,and all reads were assembled into 65604 Unigenes with an average length of 337.78 bp.The obtained sequences were annotated to NR,NT,KOG,GO and KEGG databases for comparison,and all Unigenes were annotated.The up-regulated genes were related to energy metabolism and protein translation,and the down-regulated genes were related to energy transport,which were rich in differential genes and could help to further develop their work to find their class differentiation-related genes.The study expands the genetic resources of black-winged soil termites and provides valuable biological resources for scientific research on the genetic structure of termite populations,genetic sociality,acquisition of effective molecular markers,and integrated prevention and control.