高表达OTUD3对C6星形胶质瘤细胞增殖的影响

EFFECT OF OVEREXPRESSION OF OTUD3 ON THE PROLIFERATION OF C6 ASTROGLIOMA CELLS

目的探究高表达OTUD3对C6星形胶质瘤细胞增殖的影响。方法C6胶质瘤细胞转染Myc-OTUD3质粒载体48 h后,采用Western Blot方法检测OTUD3蛋白表达变化;采用Cell Counting Kit-8(CCK-8)细胞增殖试剂盒和细胞克隆形成实验检测高表达OTUD3的C6胶质瘤细胞增殖改变情况。结果转染Myc-OTUD3质粒载体48 h后,C6胶质瘤细胞OTUD3蛋白表达升高(t=3.472,P<0.01)。CCK-8和细胞克隆形成实验结果显示,高表达OTUD3的C6胶质瘤细胞增殖水平降低(F=17.326、70.345,t=3.646,P<0.05)。结论高表达OTUD3可抑制C6星形胶质瘤细胞的增殖。

Objective To investigate the effect of overexpression of OTUD3 on the proliferation of C6 astroglioma cells.Methods Myc-OTUD3 was transfected into C6 astroglioma cells for 48 h.Western Blot was used to determine OTUD3 protein expression changes.The Cell Counting Kit-8(CCK-8)and colony-forming assays were used to measure the impact of OTUD3 overexpression on the proliferation of C6 astroglioma cells.Results After transfection with the Myc-OTUD3 vector for 48 h,OTUD3 protein expression was significantly increased in C6 astroglioma cells(t=3.472,P<0.01).The CCK-8 and colony-forming assays showed that high expression of OTUD3 significantly reduced the proliferation of C6 astroglioma cells(F=17.326,70.345;t=3.646;P<0.05).Conclusion Overexpression of OTUD3 inhibits the proliferation of C6 astroglioma cells.