摘要
目的探讨胰岛素样生长因子-1(IGF-1)与淫羊藿素(ICT)合用对脂多糖(LPS)诱导的BV2小胶质细胞炎症反应的影响。方法常规培养BV2小胶质细胞,将细胞分为对照组、LPS组、IGF-1+LPS组、ICT+LPS组和IGF-1+ICT+LPS组。其中LPS组细胞加入LPS(1 mg/L)作用6 h;后3组细胞先加入IGF-1(12.5μg/L)或(和)ICT(10μmol/L)预保护1 h,再加入LPS(1 mg/L)共同作用6 h。应用实时荧光定量PCR检测各组细胞环氧合酶-2(COX-2)和诱导型一氧化氮合酶(iNOS)mRNA的表达。结果与对照组比较,LPS组COX-2和iNOS mRNA表达明显上调(F=20.77、39.85,P<0.05);IGF-1和ICT预处理均可显著下调LPS诱导的BV2小胶质细胞COX-2和iNOS mRNA的表达,且IGF-1和ICT联合用药的下调作用比单独应用两种药物更显著(F=54.84~241.68,P<0.05)。结论IGF-1和ICT均能明显抑制LPS诱导的BV2小胶质细胞炎症反应,且二者联合应用较单独应用更有效。
Objective To investigate the effect of insulin-like growth factor-1(IGF-1)combined with icaritin(ICT)on the inflammatory response of BV2 microglial cells induced by lipopolysaccharide(LPS).Methods BV2 microglial cells were cultured with conventional methods and were then divided into control group,LPS group,IGF-1+LPS group,ICT+LPS group,and IGF-1+ICT+LPS group.The cells in the LPS group were treated with LPS(1 mg/L)for 6 hours,and those in the other three groups were pre-protected with IGF-1(12.5μg/L)and/or ICT(10μmol/L)for 1 hour,followed by the addition of LPS(1 mg/L)for 6 hours.Quantitative real-time PCR was used to measure the mRNA expression of cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)in each group.Results Compared with the control group,the LPS group had significant increases in the mRNA expression levels of COX-2 and iNOS(F=20.77,39.85;P<0.05).Pretreatment with IGF-1 and ICT significantly downregulated the mRNA expression of COX-2 and iNOS in BV2 microglial cells induced by LPS,and the combination of IGF-1 and ICT had a significantly greater downregulating effect than the two drugs used alone(F=54.84-241.68,P<0.05).Conclusion Both IGF-1 and ICT can significantly inhibit the inflammatory response of BV2 microglial cells induced by LPS,and the combination of IGF-1 and ICT is more effective than the two drugs used alone.
作者
解晓曼
胡子凡
杨妍卿
陈文芳
XIE Xiaoman;HU Zifan;YANG Yanqing;CHEN Wenfang(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)
出处
《青岛大学学报(医学版)》
2022年第3期353-356,共4页
Journal of Qingdao University(Medical Sciences)
基金
国家自然科学基金资助项目(31871144)
山东省自然科学基金资助项目(ZR2019MH018)。