细粒棘球蚴通过STAT6促进巨噬细胞向M2型极化

Echinococcus granulosus promotes macrophage polarization to M2 type via STAT6

目的:探讨细粒棘球蚴通过调控巨噬细胞STAT6的表达介导其极化的机制。方法:使用细粒棘球蚴囊液(EgCF)处理巨噬细胞系Raw264.7,以未加EgCF组作为对照组,qRT-PCR、ELISA、流式细胞术等实验检测M1、M2相关因子的表达水平,研究细粒棘球蚴对巨噬细胞极化的影响;使用EgCF处理巨噬细胞,并在实验组加入信号转导和转录激活因子6(STAT6)抑制剂,以未加抑制剂组及DMSO组作为对照组,qRT-PCR、流式细胞术等实验检测STAT6、M1、M2相关因子的表达水平,研究细粒棘球蚴通过调控巨噬细胞STAT6的表达,介导巨噬细胞极化的机制。结果:EgCF促进STAT6和M2相关因子的表达(P<0.05),抑制M1相关因子的表达(P<0.05)。STAT6抑制剂可抑制Raw264.7细胞中STAT6和M2相关因子表达,并上调M1相关因子表达。结论:EgCF通过促进STAT6的表达介导巨噬细胞向M2型极化。

Objective:To explore the mechanism of macrophage polarization mediated by Echinococcus granulosus through regulating STAT6 expression in macrophage.Methods:Echinococcus granulosuscyst fluid(EgCF)was used to treat macrophages Raw264.7,the group without EgCF was used as a comparison control group.qRT-PCR,ELISA and flow cytometry were used to detect M1 and M2-related factors in macrophages to study the effect of Echinococcus granulosus on macrophage polarization;macrophages were treated with EgCF,and an inhibitor of signal transducer and activator of transcription6(STAT6)was added to experimental group,while non-inhibitor group and DMSO group were used as control groups.qRT-PCR and flow cytometry were used to detect expressions of STAT6,M1 and M2-related factors to investigate the mechanism by which Echinococcus granulosus mediates polarization of macrophages by regulating expression of STAT6 in macrophages.Results:EgCF promoted expressions of STAT6 and M2-related factors(P<0.05)and inhibited expressions of M1-related factors(P<0.05).STAT6 inhibitor inhibited expressions of STAT6 and M2-related factors and up-regulated expressions of M1-related factors in Raw264.7 cells.Conclusion:EgCF mediates macrophage polarization towards M2 phenotype by promoting STAT6 expression.