干扰PARP1基因表达对胃癌MGC-803细胞增殖、侵袭及迁移的影响

Effects of Interfering with PARP1 Gene Expression on Proliferation,Invasion and Migration of Gastric Cancer MGC-803 Cells

【目的】探讨多聚腺苷二磷酸核糖聚合酶1(PARP1)基因对胃癌细胞(MGC-803)增殖、迁移、侵袭的影响。【方法】体外培养人胃癌细胞系MGC-803细胞和人正常胃黏膜上皮细胞GES-1,比较两种细胞PARP1 mRNA表达差异;将MGC-803细胞随机分为对照组、干扰PARP1表达组(si-PARP1组)及阴性对照组(si-NC组);qRT-PCR法检测各组MGC-803细胞PARP1 mRNA表达;MTT法、流式细胞术、划痕实验、Transwell小室法分别检测细胞增殖、凋亡、迁移与侵袭,Western blot检测PARP1、基质金属蛋白酶-2(MMP-2)、MMP-9、RhoA、Rock1蛋白表达。【结果】与GES-1比较,MGC-803细胞PARP1 mRNA表达显著升高(P<0.05);与对照组和si-NC组相比,si-PARP1组MGC-803细胞凋亡率和增殖抑制率显著升高(P<0.05),细胞划痕迁移率、侵袭率、PARP1 mRNA和PARP1蛋白、MMP-2、MMP-9、RhoA、Rock1蛋白表达水平显著降低(P<0.05)。【结论】干扰PARP1表达可抑制人胃癌细胞细胞增殖、迁移及侵袭,其可能与抑制RhoA/Rock信号通路有关。

【Objective】To investigate the effect of poly adenosine diphosphate ribose polymerase 1(PARP1)gene on the proliferation,migration and invasion of gastric cancer cell line(MGC-803).【Methods】Human gastric cancer cell line MGC-803 and human normal gastric mucosal epithelial cell GES-1 were cultured in vitro,and the expression of PARP1 mRNA was compared between the two cells;MGC-803 cells were randomly divided into control group,interference PARP1 expression group(si-PARP1 group)and negative control group(si-NC group);The expression of PARP1 mRNA in MGC-803 cells was detected by qRT-PCR;MTT assay,flow cytometry,scratch test and Transwell chamber method were used to detect cell proliferation,apoptosis,migration and invasion respectively.Western blot was used to detect the expression of PARP1,matrix metalloproteinase-2(MMP-2),MMP-9,RhoA and Rock1 proteins.【Results】Compared with GES-1,the expression of PARP1 mRNA in MGC-803 cells increased significantly(P<0.05);Compared with the control group and the si-NC group,the apoptosis rate and proliferation inhibition rate of MGC-803 cells in the si-PARP1 group increased significantly(P<0.05),and the migration rate and invasion rate of cell scratch,the expression levels of PARP1 mRNA and PARP1 protein,MMP-2,MMP-9,RhoA and Rock1 protein decreased significantly(P<0.05).【Conclusion】Interfering with the expression of PARP1 can inhibit the proliferation,migration and invasion of human gastric cancer cells,which may be related to the inhibition of RhoA/Rock signaling pathway.