2019-2020年浙江省湖州市副溶血弧菌临床分离株特征分析

Characterization of clinical isolates of Vibrio parahaemolyticus in HuZhou,Zhejiang,2019-2020

目的了解2019—2020年浙江省湖州市腹泻患者副溶血弧菌分离株的特征。方法对2019—2020年分离自腹泻患者的109株副溶血弧菌进行血清学分型,采用荧光PCR方法检测其毒力基因,采用微量肉汤稀释法检测其耐药性,并利用脉冲场凝胶电泳(PFGE)对其进行分子分型。结果109株分离株的优势血清型为O3∶K6(72株)。所有分离株均携带tlh基因,仅2株菌携带trh基因。108株菌产生72种PFGE带型,不同带型的相似系数为19.10%~100%。分离株对磺胺甲恶唑的耐药率最高(63.30%),其次为氨苄西林(49.54%)。结论加强腹泻患者副溶血弧菌分离株的持续监测有助于开展副溶血弧菌引起的食源性疾病的风险评估,为其引起的肠道疾病防控及临床治疗用药提供参考。

Objective To understand the characteristics of Vibrio parahaemolyticus isolated from diarrhea patients in Huzhou,Zhejiang province,from 2019 to 2020.Methods A total of 109 strains of V.parahaemolyticus isolated from patients with diarrhea in Huzhou from 2019 to 2020 were collected for serological typing.Virulence genes were detected by fluorescence PCR.Drug resistance was detected by broth dilution method,and molecular typing was performed by pulsed-field gel electrophoresis(PFGE).Results The predominant serotype of the 109 clinical strains was O3∶K6(72 strains).All the strains carried gene tlh,and only two of them carried trh.There were 108 strains which were divided into 72 PFGE types by NotⅠenzyme,and the similarity coefficient between different types was 19.10%–100%.The antibiotic resistance test indicated that the isolates were highly resistant to sulfamethoxazole(63.30%),followed by ampicillin(49.54%).Conclusion Strengthening the continuous surveillance for V.parahaemolyticus in diarrhea patients can facilitate the risk assessment of foodborne illnesses caused by V.parahaemolyticus and provide reference for the prevention,control and clinical treatment of intestinal infection.