甜瓜SnRK2基因家族的鉴定与特征分析

Identification and Characterization of SnRK2 Gene Family in Melon

蔗糖非发酵型蛋白激酶2(Sucrose non-fermenting-1-related protein kinase2,SnRK2)是一类具有丝氨酸/苏氨酸特定结构域的蛋白质磷酸化酶,在ABA信号介导和非生物胁迫应答方面起到重要作用。以甜瓜为研究对象,使用生物信息学分析方法,对甜瓜SnRK2基因家族进行鉴定及相关特征分析,包括构建系统发育进化树,以及基因理化性质、基因结构及保守结构域、染色体定位、共线性分析、基因的上游顺式作用元件及转录组数据热图分析等,为甜瓜SnRK2基因的功能分析和研究奠定一定的理论基础。结果表明,在甜瓜基因组中共鉴定出9个SnRK2基因家族成员。主要定位在细胞骨架中,以α-螺旋和不规则卷曲为主,分子质量为31.49~75.02 ku,等电点为4.42~6.21。其可分为3个亚族,分布在4条染色体上。启动子分析结果显示,在甜瓜SnRK2基因的调控序列中有多个植物激素和逆境胁迫相关响应元件。组织表达分析结果表明,有些甜瓜SnRK2基因在不同组织中呈组成型表达,有些基因则具有显著的组织特异性。

Sucrose non-fermenting-1-related protein kinase2(SnRK2)is a kind of protein phosphorylase with serine/threonine specific domain,which plays an important role in ABA signal transduction and abiotic stress.Taking melon as the research object,this paper used bioinformatics analysis methods to identify melon SnRK2 gene family and analyze the relevant characteristics,including the construction of phylogenetic evolutionary tree,analyses of gene physical and chemical properties,gene structure and conserved domain,chromosome location,collinearity,upstream cis-acting elements of gene and transcriptome data heat map analysis to lay a theoretical foundation for the functional analysis and research of melon SnRK2 genes.Results showed that nine SnRK2 gene family members were identified in melon genome.It was mainly located in the cytoskeleton,dominated byα-helix and random coil.The molecular weight was 31.49—75.02 ku,and the isoelectric point ranged from 4.42 to 6.21.It could be divided into three subgroups,distributed on four chromosomes.Promoter analysis showed that there were multiple plant hormones and stress-related response elements in the regulatory sequence of melon SnRK2 genes.Tissue expression analysis showed that some melon SnRK2 genes were constitutively expressed in different tissues,while some genes had significant tissue specificity.