腹腔注射GnIH对雄性小鼠采食、体重和血糖稳态的影响

Effects of Intraperitoneal Injection of GnIH on Feed Intake,Body Weight and Blood Glucose Homeostasis in Male Mice

[目的]探究促性腺激素抑制激素(gonadotropin-inhibitory hormone,GnIH)对雄性小鼠采食、体重和血糖稳态的影响。[方法]选取体重及日龄相近的雄性小鼠20只,随机分为2组,分别为对照组[腹腔注射生理盐水100μL/(次·只)]和试验组[腹腔注射20μg/100μL GnIH,100μL/(次·只)],每组10只,每天注射2次,连续注射21 d。观察和记录小鼠的采食情况;对小鼠的体重、空腹血糖、葡萄糖耐量、胰岛素耐量进行测定;利用实时荧光定量PCR (qPCR)方法对小鼠胰腺中胰岛素转录因子基因(NeuroD1)、胰岛素基因(Ins)、胰高血糖素基因(Gcg)、胰岛素转录调控因子基因(Pdx1)mRNA相对表达量进行检测。[结果]与对照组相比,试验组小鼠平均日增重和平均日采食量极显著(P<0.01)升高;试验组小鼠空腹血糖曲线下面积极显著(P<0.01)增加,葡萄糖耐量的血糖曲线下面积极显著(P<0.01)增加,胰岛素耐量的血糖曲线下面积极显著(P<0.01)增加;试验组小鼠胰腺Gcg基因mRNA相对表达量显著(P<0.05)升高,Ins基因mRNA、Pdx1基因mRNA和NeuroD1基因mRNA相对表达量均显著(P<0.05)下降。[结论]慢性腹腔注射GnIH会引起雄性小鼠采食量和体重增加,并引起机体血糖紊乱。

[Objective]To investigate the effects of gonadotropin-inhibitory hormone(GnIH)on feed intake,body weight and blood glucose homeostasis in male mice.[Method]A total of 20 male mice of similar weights and days of age were randomly divided into a control group(n=10)which received an intraperitoneal injection of 100μL physiological saline per time per mouse,and an experimental group(n=10)which received an intraperitoneal injection of 20μg/100μL GnIH with 100μL per time per mouse.The mice received the intraperitoneal injection of physiological saline or GnIH twice a day for consecutive 21 days.The feed intake of the mice were observed and recorded,and their weights,fasting blood glucose,glucose tolerance and insulin tolerance were determined.In addition,quantitative PCR(qPCR)was used to assess the mRNA relative expression levels of insulin transcription factor gene(NeuroD1),insulin gene(Ins),glucagon gene(Gcg)and insulin transcription regulative factor gene(Pdx1)in pancreas of the mice.[Result]Compared with the control group,the average daily gain and average daily feed intake of the experimental group were extremely significantly(P<0.01)higher.The area under the curve(AUC)of fasting blood glucose test,the AUC of glucose tolerance test and the AUC of insulin tolerance test of the experimental group all increased extremely significantly(P<0.01).In the experimental group,significantly(P<0.05)higher mRNA relative expression level of Gcg gene in pancreas was observed,while the significantly(P<0.05)lower mRNA relative expression levels of Ins,Pdx1 and NeuroD1 genes were found.[Conclusion]Chronic intraperitoneal injection of GnIH caused increased feed intake and body weight as well as disordered blood glucose in male mice.