黑木耳多糖的分离制备及单糖组成初探

Isolation,Preparation and Monosaccharide Composition of Auricularia auricular Polysaccharide

本文以黑木耳子实体为试验材料,运用现代分离分析技术,对碱提得到的黑木耳粗多糖(AA)进行前处理,选取DNS法和紫外可见光谱对多糖得率和多糖纯度进行鉴定,利用高效液相色谱测定其单糖组成。结果表明,黑木耳粗多糖是一种以甘露糖为主的杂多糖,单糖组分为甘露糖、氨基葡萄糖、葡萄糖、半乳糖、木糖和阿拉伯糖,各单糖组分摩尔比为1.00∶0.12∶0.53∶0.07∶0.29∶0.53(以甘露糖为基准)。

In this paper,the fruit of Auricularia auricular was used as the test material,and the crude polysaccharide of Auricularia auricular(AA)obtained by alkali extraction was pretreated by modern separation and analysis technology,and the polysaccharide yield and polysaccharide purity were identified by DNS method and UV-Vis spectroscopy,and the monosaccharide composition was determined by high performance liquid chromatography.The results show that Auricularia auricular crude polysaccharide is a heteropolysaccharide mainly composed of mannose.AA was composed of Man,GlcUA,Glc,Gal,Xyl,Ara in the ratio of 1.00∶0.12∶0.53∶0.07∶0.29∶0.53.