金花茶番茄红素β-环化酶基因的克隆及表达分析

Cloning and Expression Analysis of Lycopeneβ-cyclase Gene of Camellia Nitidissima

以金花茶花瓣为研究材料,利用同源克隆和RACE技术,克隆了金花茶LCYb基因的全长序列,命名为CnLCYb,对该基因进行生物信息学分析。结果表明:金花茶CnLCYb开放阅读框长度为1515 bp,共编码504个氨基酸,具有PLN02463、Carotene-cycl、Lycopene_cycl等保守结构域;系统进化分析发现金花茶CnLCYb蛋白与茶树LCYb蛋白的亲缘关系最近。实时荧光定量PCR分析结果表明,CnLCYb基因在金花茶开花的幼蕾期、初蕾期、显色期的表达量相对较低,在半开期、盛开期表达量较高,在花瓣中的表达总体呈上升趋势。

Using the petals of Camellia japonicae as research materials,the full length sequence of LCYb is cloned by homologous cloning and RACE technology,named CnLCYb.Subsequently bioinformatics analysis of the CnLCYb is performed,and its expression pattern is analyzed at the transcriptional and protein levels.The results show that the length of open reading frame of CnLCYb is totally 1515 bp,and encods 504 amino acids,which has many onserved domains such as PLN02463,caroteneCyCl and lycopene_CyCl.Phylogenetic analysis show that the CnLCYb protein is closely related to the LCYb protein of Camellia sinensis.The results of real-time fluorescence quantitative PCR analysis show that the expression level of CnLCYb is relatively low in young bud,early bud and chromogenic stages of flowering of Camellia nitidissima,but high in the half-opening and blooming stage,and the expression pattern of CnLCYb in the petals shows an overall upward trend.