梯度营养稀释法筛选贫营养净水微生物的研究

Study on screening water oligotrophic bacteria by gradient nutrient dilution method

应用贫营养微生物去除微污染水体中的低浓度溶解性污染物,是近年来给水处理领域一个新兴的研究方向。研究采用梯度营养稀释法,将从富营养培养基分离出来的菌株依次接种于不同稀释倍数的贫营养培养基上,从而筛选出兼性贫营养净水微生物。结果表明,从淮安市二河水源地水体和底泥中分离出的2株高效的兼性贫营养菌株分别为短小芽孢杆菌(Bacillus pumilus)、蜡样芽孢杆菌(Bacillus cereus),其对低浓度水体中的7日TOC降解率均可达到60%以上。2种菌株均能利用多种碳源和氮源生长,浓度为3%的双氧水和10%的乙醇均能将菌株杀死,可安全有效地应用于给水处理领域中。

The application of water oligotrophic bacteria to the removal of low-concentration dissolved pollutants from slightly polluted water is a new research direction in the field of water supply treatment.The bacteria isolated from the eutrophic medium were inoculated on the oligotrophic medium with different dilution ratios,so as to screen out the facultative oligotrophic microorganisms.The results showed that Bacillus pumilus and Bacillus cereus were the two highly effective bacteria isolated from the water and sediment of the Second River in Huai’an,and the TOC in the low-concentration water could be removed more than 60%7 days after dosing the stains.Both strains could use a variety of carbon and nitrogen sources to grow,and the concentration of 3%hydrogen peroxide and 10%ethanol could kill strains,Therefore,both of them could be safely and effectively used in the field of water treatment.