PRAS40mRNA高表达对乳腺癌的诊断及预后评估的价值分析

Prognostic and Diagnostic Role of High PRAS40 mRNA Expression in Breast Cancer

目的分析乳腺癌患者中PRAS40mRNA表达量与其预后的相关性。方法通过从TCGA数据库中下载PRAS40mRNA的表达矩阵进行分析。受试者工作曲线(ROC曲线)被用来分析PRAS40mRNA表达量在乳腺癌患者中的诊断价值。使用卡方检验分析临床特征与PRAS40mRNA表达量之间的相关性。Cox回归分析PRAS40mRNA表达量与其预后的关系。结果结果显示:乳腺癌组织PRAS40mRNA表达量较正常组织表达量高。PRAS40具有较强诊断价值。PRAS40mRNA表达量与T3/T4分期患者高于T1/T2分期(P<0.05);浸润性癌患者其表达水平高于浸润性小叶癌(P<0.01);病理分期StageⅠ/Ⅱ分期的患者相较于StageⅢ/Ⅳ期患者表达水平低(P<0.05)。但是不同N分期及M分期患者PRAS40mRNA表达水平无明显统计学差异。进一步多变量分析显示:PRAS40mRNA表达水平是乳腺癌患者的独立危险因素。结论PRAS40mRNA是1个可靠的且具有预后价值的生物标记物。

Objective To analyze the correlation between PRAS40 mRNA expression and prognosis in breast cancer patients.Methods The PRAS40 mRNA expression using the data from TCGA database were analyzed.A receiver operating characteristic(ROC)curve was performed to assessed the diagnostic value of PRAS40 mRNA expression in breast cancer.Chi-square test was used to analyzed the correlation between clinical characteristics and PRAS40 mRNA expression.Cox analysis were performed to determine the prognostic value of PRAS40 mRNA expression in breast cancer.Results PRAS40 mRNA expression was higher in breast tissues than in normal tissues.PRAS40 presented a moderate diagnostic value in breast cancer.PRAS40 mRNA expression was associated with higher expression levels in patients with T3/T4 stage than T1/T2 stage(P<0.05);higher expression levels in patients with histology of invasive carcinoma than invasive lobular carcinoma(P<0.01);and lower expression levels in patients with StageⅠ/Ⅱstage compared with StageⅢ/Ⅳstage(P<0.05).High PRAS40 mRNA expression was associated with poor overall survival.However,no statistically significant differences in PRAS40 mRNA expression levels were seen in patients with different N or M stages.Furthermore,Multivariate analysis revealed that PRAS40 was an independent risk factor for breast cancer patients.Conclusion PRAS40mRNA is a reliable and prognostically valuable biomarker.