负载BMP-2和VEGF的3D打印多孔钛支架修复颌骨缺损的实验研究

The experimental study of 3D printed porous titanium scaffold loaded with BMP-2 and VEGF for repairing jaw defect

目的初步探讨负载细胞因子的三维打印多孔钛合金支架的成骨能力。方法利用CT数据设计颌骨缺损形态及大小并建模。使用激光选区熔化技术对Ti6Al4V粉末进行加工制作出多孔支架。检测其孔隙率及不同处理状态下的塑形性。通过负载不同比例的BMP-2/VEGF修复兔颌骨缺损。标本硬组织切片后,亚甲基蓝-酸性品红染色评估成骨效果。结果经800℃/2 h热处理后塑形性增加。一定浓度的BMP-2及VEGF能够加速新生骨在三维打印多孔钛合金支架上的形成。但呈现出非线性剂量依赖性。结论采用激光选区熔化技术制作的Ti6Al4V多孔支架作为骨缺损替代材料是初步可行的,负载一定浓度的BMP-2及VEGF有加速成骨的作用。

Objective To vestigate the bone-forming ability of three-dimensional printed porous titanium scaffold loaded with cytokines.Methods CT data was used to design the jaw defect.The Ti6Al4V powder was processed using selective laser melting technology to produce the porous scaffold.The porosity of the scaffold and plasticity in different processing states were detected.Porous scaffolds loaded with different proportion of BMP-2/VEGF were used to repair rabbit jaw defect.After undecalcified bones slicing,methylene blue-acid magenta staining was used to evaluate the osteogenic effect.Results The plasticity increased after heat treatment at 800℃/2 h.The formation of new bone in the porous titanium scaffold could be accelerated by a certain concentration of BMP-2 and VEGF.Conclusions It is initially feasible to make Ti6Al4V porous scaffold as an alternative material for bone defect by selective laser melting technology.The BMP-2 and VEGF with a certain concentration have the effect of accelerating osteogenesis.