胃癌患者血浆PAX5和RNF180基因甲基化情况及其临床意义

Methylation of plasma PAX5 and RNF180 genes in patients with gastric cancer and its clinical significance

目的研究胃癌患者血浆配对盒蛋白5(PAX5)、环指蛋白180(RNF180)基因甲基化情况及其临床意义。方法采用回顾性分析方法,研究对象为2018年1月至2019年6月南京鼓楼医院集团宿迁医院入院的55例胃癌患者,将其设为研究组,同时选取同一时期入院的25名体检健康者设为对照组。选取甲基化特异性聚合酶链式反应(PCR)法测定两组血浆PAX5、RNF180基因甲基化状态,并分析其与胃癌患者病理特征的相关性;随访3年,选取Kaplan-Meier生存曲线分析胃癌患者预后与PAX5、RNF180甲基化的相关性。结果研究组血浆PAX5、RNF180基因启动子区甲基化发生率明显高于对照组,差异均有统计学意义(P<0.05)。研究组TNM分期Ⅰ~Ⅱ期患者的血浆PAX5、RNF180基因甲基化发生率明显低于Ⅲ~Ⅳ期患者,差异均有统计学意义(P<0.05)。研究组内男性患者与女性患者、肿瘤直径<2.5 cm患者与≥2.5 cm、年龄<55岁患者与≥55岁患者、淋巴结转移患者与无淋巴结转移患者、细胞低-中分化患者与高分化患者的血浆PAX5、RNF180基因甲基化发生率比较,差异无统计学意义(P>0.05)。未出现PAX5基因甲基化胃癌患者3年生存率明显高于出现甲基化的患者,差异有统计学意义(P<0.05)。未出现RNF180基因甲基化胃癌患者3年生存率与出现甲基化患者相比,差异无统计学意义(P>0.05)。结论胃癌患者血浆PAX5、RNF180基因甲基化发生风险明显高于健康人群,与肿瘤TNM分期关系密切,且PAX5基因甲基化可致使胃癌患者生存率下降。

Objective To study the methylation of plasma pECTIN 5(PAX5)and ring finger protein 180(RNF180)in patients with gastric cancer and its clinical significance.Methods Retrospective analysis method was used,the research subjects were 55 patients with gastric cancer who were admitted to Nanjing Gulou Hospital Group suqian Hospital from January 2018 to June 2019.They were set as the research group,and 25 healthy people who were admitted to the hospital during the same period were selected as the control group.The methylation-specific PCR method was used to determine the methylation status of plasma PAX5 and RNF180 genes in the two groups,and its correlation with the pathological characteristics of gastric cancer patients was analyzed.After 3 years of follow-up,the correlation between the prognosis of gastric cancer patients and the methylation of PAX5 and RNF180 was analyzed and the Kaplan-Meier survival curve was selected.Results The incidence of methylation in the promoter regions of PAX5 and RNF180 genes in the study group was significantly higher than that in the control group,and the difference was statistically significant(P<0.05).The incidence of plasma PAX5 and RNF180 gene methylation in patients with TNM stageⅠ-Ⅱin the study group was significantly lower than that in patients with stageⅢ-Ⅳ,and the difference was statistically significant(P<0.05).Male patients and female patients in the study group,patients with tumor diameter<2.5 cm and≥2.5 cm,patients aged<55 years and patients≥55 years old,patients with lymph node metastasis and patients without lymph node metastasis,patients with low-moderately differentiated cells and patients with high differentiation There was no significant difference in the incidence of plasma PAX5 and RNF180 gene methylation between the two groups(P>0.05).The 3-year survival rate of gastric cancer patients without PAX5 gene methylation was significantly higher than that of patients with methylation(P<0.05).There was no significant difference in the 3-year survival rate of gastric cancer patients without RNF180 gene methylation compared with those with methylation(P>0.05).Conclusion The risk of plasma PAX5 and RNF180 gene methylation in gastric cancer patients was significantly higher than that in healthy people,and was closely related to tumor TNM staging,and PAX5 gene methylation could reduce the survival rate of gastric cancer patients.