水产品腐败希瓦氏菌双组分信号转导系统的全基因组鉴定与分析

Genome-wide Identification and Analysis of Two-component Signal Transduction Systems in Shewanella putrefaciens from Aquatic Products

腐败希瓦氏菌是水产品中的一种特定腐败菌,具有较强的低温适应性和致腐性。双组分信号转导系统(TCSs)是细菌中重要的调控系统之一,在细菌对复杂环境的感知和响应中起重要的作用。为探究TCSs在腐败希瓦氏菌中的分布及功能,本文采用全基因组鉴定、多序列比对、系统进化树分析、蛋白结构预测以及互作蛋白分析等多种生物信息学方法,系统分析水产品腐败希瓦氏菌中TCSs的分布、分类、结构与功能。结果表明:水产品腐败希瓦氏菌WS13菌株中存在31个组氨酸激酶(HKs)、36个反应调节蛋白(RRs)、3个成对TCSs、3个融合HKs。HKs共有16种结构域组成形式,其中15种均含有HATPase_c激酶催化结构域。RRs具有10种结构域组成,均含有REC结构域。多序列比对和进化分析表明:不同结构域的HKs和RRs具有不同的进化关系,可能存在功能的多样性。3对TCSs和3个融合HKs的生物学功能主要参与响应信号刺激,调控细胞内的各种代谢过程以及物质的运输等。3个融合HKs均与三甲胺生成相关。研究结果为进一步探究TCSs在水产品腐败希瓦氏菌中的功能提供理论参考。

Shewanella putrefaciens is a kind of specific spoilage organism of aquatic products,which possess strong cold adaptability and spoilage ability.Two-component signal transduction systems(TCSs)is one of the most important regulatory systems in bacteria,and plays an important role in the perception and response of bacteria to complex environment.In order to explore distribution and function of TCSs in S.putrefaciens.In this paper,the distribution,classification,structure and function of TCSs in S.putrefaciens from aquatic products,were systematically analyzed by bioinformatics methods,including whole genome identification,multiple sequence alignment,phylogenetic analysis,protein structural prediction and interaction protein analysis.The results showed that there were 31 histidine kinases(HKs),36 response regulators(RRs),3 pairs of TCSs and 3 fusion HKs in S.putrefaciens WS13.There are 16 types of structural domain compositions in HKs,15 of which possess HATPase_c kinase catalytic domains.RRs have10 types of structural domain compositions,all of which contain REC domains.Multiple sequence alignment and evolutionary analysis showed that HKs and RRs with different structural domains had different evolutionary relationships,which suggested functional diversity of these proteins.The biological functions of 3 pairs of TCSs and 3 fusion HKs were predicted to be mainly involved in response to signal stimulation,regulation of various metabolic processes and transport of substances in cells.Moreover,3 fusion HKs were related to the formation of trimethylamine.The results provide references for further exploring the function of TCSs in S.putrefaciens from aquatic products.