西藏黄绿卷毛菇生境土壤微生物群落组成

Composition of soil microbial community in the habitat of Floccularia luteovirens in Tibet,southwest China

牧草外生菌根菌黄绿卷毛菇Floccularia luteovirens具有较高的生态和经济价值,其生境微生物对其菌丝生长、菌根化和子实体形成具有促进作用。本研究利用高通量测序技术对西藏黄绿卷毛菇菌窝土壤及其周围无菇土壤微生物群落组成进行分析,挖掘促进黄绿卷毛菇生长的有益微生物资源。结果显示,西藏黄绿卷毛菇生境土壤细菌隶属于17门22纲116目161科227属,其中酸杆菌门、变形菌门、拟杆菌门和疣微菌门累计比例高达81.75%;生境土壤真菌隶属于5门17纲34目55科61属,子囊菌门和担子菌门累计比例高达80.89%。对比周围无菇土壤的微生物群落,促进黄绿卷毛菇生长的潜在细菌类群为Flavisolibacter、黄杆菌属Flavobacterium、芽单胞菌属Gemmatimonas、Haliangium、赛格特杆菌Segetibacter和鞘氨醇单胞菌属Sphingomonas;促进黄绿卷毛菇生长的潜在真菌类群为斜盖伞属Clitopilus、丝膜菌属Cortinarius、被孢霉属Mortierella和毛霉属Mucor。土壤微生物促进黄绿卷毛菇生长繁殖的机制有待进一步研究。

Floccularia luteovirens,an ectomycorrhizal fungus in grassland,is highly valuable to ecology and economy.Herbage rhizosphere microorganisms can promote the growth of ectomycorrhizal fungi,mycorrhizal formation and the formation of fruiting bodies.In this study,high-throughput sequencing technology was applied to analyze the microbial community composition in fruiting body-emersed and surrounding soil of F.luteovirens in Tibet,attempting to explore beneficial microorganism resources for promoting the fungal growth.The results showed that bacteria in the soil of F.luteovirens habitat could be referred to 17 phyla,22 classes,116 orders,161 families and 227 genera,and the dominant phyla Acidobacteria,Proteobacteria,Bacteroidetes and Verrucomicrobia accounted for 81.75%.Fungi in the habitat soil were referred to 5 phyla,17 classes,34 orders,55 families and 61 genera,and dominant phyla Ascomycota and Basidiomycota accounted for 80.89%.The bacteria potentially promoting the growth of F.luteovirens were Flavisolibacter,Flavobacterium,Gemmatimonas,Haliangium,Segetibacter and Sphingomonas;the fungi potentially promoting the growth of F.luteovirens were Clitopilus,Cortinarius,Mortierella and Mucor.The mechanisms of soil microorganisms promoting the growth and reproduction of F.luteovirens need further study.