不同银杏内酯对黑曲霉线粒体结构及功能的影响

Effects of different ginkgolides on mitochondrial structure and function of Aspergillus niger

真菌感染作为威胁人类健康的传染性疾病之一,是普遍的全球性问题。本研究通过在黑曲霉的孢子悬浮液中添加不同浓度的银杏内酯,收集黑曲霉菌丝体,评估黑曲霉线粒体的结构和功能,考察不同银杏内酯B和C对黑曲霉的抗真菌效果。透射电子显微镜扫描发现经银杏内酯B处理后的黑曲霉线粒体表现出明显的空泡化,基质结构被破坏;线粒体三羧酸循环中关键酶琥珀酸脱氢酶和苹果酸脱氢酶活性下降,线粒体膜电位降低,丙二醛和活性氧水平显著升高。研究结果初步揭示了银杏内酯以剂量依赖的方式损坏线粒体膜的完整性,破坏线粒体结构,进一步损害线粒体功能;银杏内酯B的抗黑曲霉效果要显著高于银杏内酯C。

As one of the infectious diseases threatening human health, fungal infection is an universal problem. The present study sought to examine how ginkgolide B and C impacts mitochondrial structure and overall functionality in Aspergillus niger. Ginkgolide with different concentrations were added to spore suspension of A. niger, then the mycelia were collected, and the antifungal effect of ginkgolides on A. niger was investigated. Observation by transmission electron microscopy showed that ginkgolide B caused significant vacuolization of mitochondria and destruction of matrix structure, resulting in the decrease of activities of succinate dehydrogenase and malate dehydrogenase, the key enzymes in mitochondrial tricarboxylic acid cycle, the reduction of mitochondrial membrane potential, and marked elevation of malondialdehyde levels. The results preliminarily revealed that ginkgolide damaged the integrity of mitochondrial membrane and mitochondrial structure, and thereby further damaged mitochondrial function in a dose-dependent manner, so as to achieve the effect against A. niger.The anti-A. niger effect of ginkgolide B was significantly higher than that of ginkgolide C.