杨树桑黄饮片水提物对免疫低下小鼠的免疫功能的影响

Effects of Sanghuangporus vaninii water extract on immune function of immunocompromised mice

探究桑黄饮片水提物对环磷酰胺所致免疫低下小鼠的免疫功能的影响。本研究以桑黄饮片为原料,采用超声和沸水提取方法得到桑黄饮片水提物,注射环磷酰胺造成小鼠免疫功能低下,通过测定脾脏和胸腺指数、血清免疫球蛋白和细胞因子、脾淋巴细胞增殖、碳粒廓清等实验,对免疫低下小鼠的免疫功能进行研究评价。结果表明,灌胃桑黄饮片水提物的小鼠与模型组相比,免疫器官指数、血清中免疫球蛋白含量、细胞因子水平、脾淋巴细胞增殖程度、巨噬细胞的吞噬速率和吞噬指数、小鼠耳肿胀度均有提高,脾脏苏木精-伊红(HE)染色也表明桑黄饮片水提物可以改善环磷酰胺导致的脾脏组织病理状态。本研究表明桑黄饮片水提物对环磷酰胺所致的免疫抑制小鼠具有良好的免疫保护作用,为桑黄饮片扩大临床应用提供实验依据。

This study aims at exploring the effects of Sanghuangporus vaninii water extract on immune function of immunocompromised mice caused by cyclophosphamide.S.vaninii water extract was obtained by using ultrasound and boiling water extracting methods.Injection of cyclophosphamide was conducted to cause immunocompromise of mice.The immune function of immunocompromised mice was evaluated by measuring spleen and thymus index,serum immunoglobulin and cytokine,splenic lymphocyte proliferation and carbon clearance.The results showed that compared with the model group,the immune organ index,serum immunoglobulin content,cytokine level,splenic lymphocyte proliferation degree,phagocytosis rate and phagocytosis index of macrophages,and ear swelling degree of mice were increased without exception in mice administered with water extract of S.vaninii.HE staining of spleen also showed S.vaninii decoction could improve the pathological state of spleen caused by cyclophosphamide.The facts proved that the water extract of S.vaninii had a good immune protection effect on immunosuppressive mice induced by cyclophosphamide,and clinical application of S.vaninii decoction seems expectable.