甜菜NAC基因家族生物信息学分析

Bioinformatics Analysis of NAC Gene Family in Sugarbeet

NAC转录因子家族是植物特有的一类重要转录因子,广泛参与植物生长、发育以及器官的建成、逆境胁迫应答等过程.为进一步研究甜菜(Beta vulgris L.)NAC转录因子家族,本研究利用生物信息学方法对甜菜NAC基因所编码的蛋白理化性质、二级结构、保守基序、亚细胞定位、系统进化关系进行了分析.结果显示,甜菜NAC转录因子序列的鉴定出52个甜菜NAC基因,不均匀的分布于9条染色体上,6号染色体上分布最多,为11个;其次是1号染色体,为10个;7号染色体分布最少,只有1个.有1个NAC转录因子成员(BvNAC30)的核苷酸序列具有miR164靶序列识别位点.甜菜NAC转录因子的进化树分析结果显示,BvNAC蛋白序列可分为18个亚族.各亚族成员数量分布差异很大,其中Ⅻ亚族的Bv NAC转录因子成员最多为28个,而Ⅸ和Ⅹ亚族成员均只有一个.其中的Ⅻ亚族与拟南芥并没有相聚成簇,甜菜NAC转录因子的NAC保守结构域是高度保守的.NAC保守结构域中包含15个亚结构域,甜菜NAC转录因子Ⅰ、Ⅴ、Ⅺ亚族成员的保守性最高,而Ⅻ亚族成员保守性最低.本研究将为甜菜NAC基因家族的进一步功能分析奠定重要的研究基础.

NAC transcription factor family is a kind of important transcription factors unique to plants.It is widely involved in plant growth,development,organ formation,stress response and so on.In order to further study the sugar beet(Beta vulgris L.)NAC transcription factor family,the physical and chemical properties,secondary structure,conserved motif,subcellular localization and phylogenetic relationship of the protein encoded by sugar beet NAC gene were analyzed by bioinformatics method.The results showed that 52 sugar beet NAC genes were identified by the sequence of sugar beet NAC transcription factor,which were unevenly distributed on 9 chromosomes,and the most distributed on chromosome 6 was 11;The second is chromosome 1,with 10;Chromosome 7 has the least distribution,only one.The nucleotide sequence of one NAC transcription factor member(bvnac30)has miR164 target sequence recognition site.Phylogenetic tree analysis of sugar beet NAC transcription factors showed that bvnac protein sequences could be divided into 18 subfamilies.The distribution of the number of members of each subfamily varies greatly.Among them,the BV NAC transcription factor members of subfamily Ⅻ are up to 28,while there is only one member of subfamilies Ⅸ and Ⅹ.The Ⅻ subfamily is not clustered with Arabidopsis thaliana,and the NAC conserved domain of sugar beet NAC transcription factors is highly conserved.The NAC conserved domain contains 15 subdomains.The members of subfamilies Ⅰ,Ⅴ and Xi of sugar beet NAC transcription factors have the highest conservation,while the members of subfamily Ⅻ have the lowest conservation.This study will lay an important research foundation for the further functional analysis of sugar beet NAC gene family.