基于新冠病毒荧光检测的RT-qPCR温控系统

The RT-qPCR temperature control system based on COVID-19 fluorescence detection

实时荧光定量聚合酶链式反应是新型冠状病毒COVID-19快速检测的重要手段,病毒样本经过一系列复杂的化学反应处理后通过RT-qPCR温控系统得到检测结果,RT-qPCR温控仪实际就是一台精准的测温、控温仪和光谱检测设备。采用ARM处理器+H桥驱动器+帕尔贴的设计架构,通过归一化PID算法解决温度的快速升降问题,从而构建低成本、小型化的温控系统。该系统可以根据不同的温度设定和荧光剂选择适用其他病毒的核酸检测,整体控温指标可以达到升温速度不小于15℃/s、降温速度不小于8℃/s、温度精度0.1℃以内、升降温过冲小于4℃,稳定时间3 s。

Real time fluorescence quantitative polymerase chain reaction is an important means of rapid detection of new coronavirus COVID-19.After sampling a series of complex chemical reactions, the virus results were obtained through the RT-qPCR temperature control system. The RT-qPCR temperature controller is actually an accurate temperature controller and spectral detection electronic equipment. This paper adopts the design architecture includedARM processor, H-bridge driver and Peltier, meanwhile realizes the rapid rise and fall of temperature through normalized PID algorithm.So as to build a low-cost and miniaturized temperature control system. The system can select nuclear acid detection suitable for other viruses according tothe different temperature settings and fluorescent agents, and the temperature control can achieve the heating rate of not less than 15 ℃/s The cooling rate shall not be less than 8 ℃/s, the temperature accuracy shall be within0.1 ℃, the overshoot of temperature rise and fall shall be less than 4 ℃, and the stability time shall be 3 s.