铁死亡主要检测方法及其应用的研究进展

Research progress on the detection methods and their application in ferroptosis

铁死亡是一种在形态学、生物化学、基因学等方面均不同于细胞凋亡、坏死、焦亡和自噬等传统细胞死亡途径的新型细胞死亡类型,其主要特征为铁累积和脂质过氧化,铁死亡的调控机制涉及铁代谢、谷胱甘肽代谢和脂质过氧化反应等方面,并与肿瘤、衰老、神经退行性疾病、缺血再灌注损伤、心脑血管疾病、肾脏损伤、肝纤维化等疾病的病理过程密切相关。如何有效研究铁死亡调控机制治疗疾病已成为铁死亡研究领域的热点。近年来,随着对铁死亡研究的深入,铁死亡的识别、确认及机制等方面的研究进展显著,用于检测铁死亡的方法不断发展且日趋成熟,形态学、生物化学、分子生物学、基因学等方面的技术已广泛应用于铁死亡的检测与研究。为进一步加深读者对铁死亡及其检测方法的认识,本文主要综述了目前常规使用的铁死亡检测方法及其应用,并对其在铁死亡检测中的优缺点进行总结和讨论。这些方法将有助于更好地理解和研究铁死亡的诱导和调控过程及其在治疗疾病中的作用。

Ferroptosis is a novel type of cell death,which is distinguished from the traditional cell death pathways such as apoptosis,proptosis,necrosis and autophagy in terms of morphology,biochemistry and genetics.The main features of ferroptosis are the iron accumulation and lipid peroxidation.The regulation mechanism of ferroptosis involves glutathione metabolism,lipid peroxidation reactions and iron metabolism,which are closely related to the pathological process of tumor,aging,neurodegenerative diseases,ischemia reperfusion injury,cardiovascular and cerebrovascular diseases,kidney injury,hepatic fibrosis and so on.How to effectively study the role of ferroptosis regulation mechanism in the treatment of diseases becomes the hot spot and focus of the ferroptosis research.In recent years,with the in-depth study of ferroptosis,the identification,confirmation and the mechanism of ferroptosis have been developed significantly and have come forth continuously,in the meantime,techniques based on the morphology,biochemistry,molecular biology and genetics have been widely applied in the detection of ferroptosis.In order to deepen readers’understanding of ferroptosis and its detection methods,this paper will mainly review the current research progress on the detection methods and their application in ferroptosis,summarize and discuss their advantages and disadvantages in the detection of ferroptosis,this knowledge are crucial for better understanding and studying the biological function of ferroptosis.