壮药红花青藤中抑制NO生成的活性成分研究

The NO inhibitory constituents from Illigera rhodantha

采用硅胶、ODS柱色谱、Sephadex LH-20等分离方法对红花青藤藤茎醇提物的化学成分进行分离纯化,通过其理化性质及波谱数据对分离得到的化合物进行结构表征。从红花青藤藤茎醇提物中共分离得到16个化合物:(2R,3R)-2,3-dihydroxy-2-methylbutane-1,4-diyldibenzoate(1)、反式-阿魏酸对羟基苯乙酯(2)、4-O-benzoyl-2-C-methyl-D-erythritol(3)、N-反式阿魏酰基-3-甲基多巴胺(4)、tribulusamide A(5)、柳杉二醇(6)、teuclatriol(7)、齐墩果酸(8)、icario A2(9)、香草酸(10)、对羟基苯甲酸(11)、没食子酸(12)、没食子酸乙酯(13)、大黄酚(14)、D-1-O-甲基-肌醇(15)和十六烷酸(16)。其中1为新化合物,其绝对构型采用Mosher法并结合ROESY法确定;除10、11和14外,其他化合物均是首次从青藤属中分离得到。与阳性对照药吲哚美辛相比,化合物4~6、8和9对LPS诱导RAW264.7细胞生成NO均有显著的抑制作用。

Sixteen compounds were isolated from the ethanol extract of Illigera rhodantha by silica gel,ODS,and Sephadex LH-20 column chromatographies.These compounds were identified as(2R,3R)-2,3-dihydroxy-2-methylbutane-1,4-diyldibenzoate(1),p-hydroxyphenethyl trans-ferulate(2),4-O-benzoyl-2-C-methyl-D-erythritol(3),N-trans feruloyl-3-methyldopamine(4),tribulusamide A(5),cryptomeridiol(6),teuclatriol(7),oleanolic acid(8),icario A2(9),vanillic acid(10),p-hydroxybenzoic acid(11),gallic acid(12),ethyl gallate(13),chrysophanol(14),D-1-O-methyl-inositol(15),and hexadecanoic acid(16)based on their spectral data and physico-chemical properties.Compound 1 is an undescribed compound,of which its absolute configurations were determined by Mosher and ROESY methods;all the compounds except 10,11 and 14 were isolated from Illigera genus for the first time.Compared with the positive control indomethacin,compounds 4-6,8 and 9 inhibited significantly against the NO production in LPS-induced RAW 264.7 cells.