大孔树脂串联富集沙漠肉苁蓉苯乙醇苷的工艺

The Process of Enrichment of Cistanche deserticola Phenethanol Glycosides in Series with Macroporous Resin

主要研究大孔吸附树脂纯化肉苁蓉苯乙醇苷类的最佳工艺条件。通过单因素试验、正交试验优化肉苁蓉有效成分提取工艺;用大孔树脂串联的方法富集有效成分。肉苁蓉苯乙醇苷类最佳提取工艺:以70%乙醇为提取溶液,在温度70℃下提取3次,每次2 h,料液比1︰10(g/L)。肉苁蓉苯乙醇苷类最佳富集工艺:用AB-8和ADS-7型大孔树脂串联的方法富集有效成分,使用AB-8型大孔树脂富集树脂与药材质量比1︰1上样,50%乙醇洗脱液;使用ADS-7型大孔树脂进行二次富集树脂与药材质量比4︰1上样,50%乙醇洗脱。该工艺简单可行,分离效果好,能很好地保留有效成分并且降低色素含量,适用于肉苁蓉有效成分的连续工业化生产。

The optimal process condition for the purification of Cistanche deserticola phenethanol glycosides by macroporous adsorption resin was mainly studied.The extraction process of the effective ingredients of Cistanche deserticola was optimized by single factor experiment and orthogonal experiment.The effective ingredients were enriched by the method of series macroporous resin.The best extraction process of Cistanche deserticola phenethyl alcohol glycosides was 70%ethanol as the extraction solution,extracted three times at a temperature of 70℃,each time for 2 h,and the ratio of material to liquid 1︰10(g/L).The best enrichment process of Cistanche deserticola phenethanol glycosides was that the active ingredients were enriched by series method of AB-8 and ADS-7 macroporous resin.Use the AB-8 macroporous resin enrichment resin and medicinal quality ratio 1︰1 to load the sample,and use 50%ethanol eluent;The ADS-7 type macroporous resin was used for secondary enrichment resin and medicinal material quality ratio 4︰1,and 50%ethanol elution.The process is simple and feasible;The separation effect is good;The active ingredients are well preserved and the pigment content is reduced;And it is suitable for continuous industrial production of effective ingredients of Cistanche deserticola.