响应面法优化藜麦蛋白提取工艺

Optimization of Extraction Technology of Quinoa Protein

采用超声波粉碎和碱法提取相结合的方法提取藜麦蛋白,通过单因素试验得出最佳工艺条件,并以响应面法进一步优化,得出实际操作过程中的最佳提取工艺参数:超声功率200 W、pH 9.5、料液比1︰25(g/mL)、提取时间40 min,此时的藜麦蛋白提取率可达75.24%。与常规碱法提取藜麦蛋白相比,超声辅助碱提取法能够显著提高藜麦蛋白提取率。

Quinoa protein was extracted by ultrasonic grinding and alkali extraction.The optimum technological condition was obtained by single factor experiment,and further optimized by response surface methodology.The optimal extraction parameters in the actual operation were as follows:ultrasonic power 200 W,pH 9.5,solid-liquid ratio 1︰25(g/mL)and extraction time 40 min.At this time,quinoa protein extraction rate could reach 75.24%.Compared with the conventional alkali extraction method,the ultrasonic-assisted alkali extraction method could significantly improve the extraction rate of quinoa protein.