HP-20大孔吸附树脂纯化白薇粗多糖

The Purification Characteristics of Crude Polysaccharide Extract from Cynanchum atratum Bunge by HP-20 Macroporous Resin

研究大孔吸附树脂对白薇粗多糖同时脱色素和脱蛋白质的最佳条件及其对多糖结构特征的影响。以色素清除率、蛋白质清除率和多糖保留率为基本指标,从7种树脂中筛选到最佳树脂类型,为HP-20。对吸附条件进行优化,结果表明多糖初始浓度5 mg/mL、初始pH 5、温度35℃、流速2.5 BV/h时,具有较好的脱色(清除率约67%)和脱蛋白质(清除率约73%)效果,且多糖损失较少(约27%)。UV、HPLC-ELSD、PMP-HPLC-DAD、1H NMR分析结果显示,吸附前后,多糖在分子量分布、单糖组成、1H NMR特征等都未发生明显改变,表明HP-20的吸附作用不会引起白薇多糖链的降解和结构改变。所获得的方法可用于白薇根粗多糖的精制和工业化生产,且为白薇根粗多糖后续分离纯化和药理活性研究奠定良好基础。

Macroporous adsorption resin was screened,and the optimal condition for simultaneous decoloration and deproteinization of crude polysaccharides from Cynanchum atratum Bunge as well as the effects on the structural characteristics of polysaccharides by the screened resin were tested and discussed.The best resin type was screened from 7resins as HP-20 based on the decoloration and deproteinization efficiency and the polysaccharide recovery rate as indicators.The adsorption condition was optimized,and the results showed that the HP-20 resin exhibited the best depigmentation rate(a removal rate of about 67%),in addition to deproteinization rate(a removal rate of about 73%),and a less polysaccharide loss(about 27%)with the initial polysaccharide concentration of 5 mg/mL,the initial pH of 5,the temperature of 35℃,and the flow rate of 2.5 BV/h.The partial structural characteristics including the molecular weight distribution,monosaccharide composition,1H NMR characteristics of polysaccharides before and after adsorption did not change significantly after analyzed using UV,HPLC-ELSD,PMP-HPLC-DAD and 1H NMR methods,and the results indicated that the adsorption of HP-20 could not cause the degradation and structural changes of the polysaccharide chains.The obtained method could be used for the refining and industrial production of the crude polysaccharides.At the same time,it laid a good foundation for the subsequent separation,purification and pharmacological activity research of the polysaccharides.