响应面法优化香榧假种皮黄酮提取及活性研究

A Study on the Extraction Process and Extraneous Activity of Flavonoids from Torreya grandis Aril

以香榧假种皮为原料,总黄酮得率为响应值,利用响应面法优化黄酮提取工艺,并测定其抗氧化和抗糖活性。结果表明,响应面法优化得到的最佳提取工艺为超声时间25.75 min、超声功率409.74 W、液料比45.88︰1(mL/g)、乙醇体积分数59.86%,总黄酮得率最高可达为15.30 mg/g。提取所得总黄酮对DPPH、ABTS有较好的清除作用;对α-葡萄糖苷酶、α-淀粉酶有一定抑制效果,证实香榧总黄酮有较好的抗氧化能力与一定的降血糖能力,可进一步开发利用。

The Torreya grandis arils were used as raw materials and its yield of total flavonoids was taken as the response value.Optimize the extraction process of total flavonoids by the response surface method,and investigate its antioxidant capacity and anti-glycemic ability.The results showed that the optimal extraction process optimized by response surface methodology was as follows:the ultrasonic time 25.75 min,the ultrasonic power 409.74 W,the liquid-to-material ratio 45.88︰1(mL/g),and the ethanol concentration 59.86%.Under this condition,the extraction rate of total flavonoids was 15.30 mg/g.In addition,free radical scavenging tests were conducted on DPPH and ABTS and the determination ofα-glucosidase andα-amylase inhibition rate,which confirmed that the total flavonoids of Torreya grandis aril had good antioxidant capacity and certain hypoglycemic ability.