黄曲霉毒素B1光纤检测系统设计

Design of optical fiber detection system for aflatoxin B1

为了解决传统化学发光酶免疫分析方法中大尺寸光电检测装置探测头与反应孔的对准难题,采用多模光纤结合光纤型光子计数探头对目标信号进行探测,设计一种基于光电检测技术的黄曲霉毒素B1光纤检测系统。通过ZEMAX仿真与测试结果显示,在反应孔范围内,与反应孔中心位置检测相比光纤光子收集比达到95.83%以上,检测相对标准偏差RSD≤0.21%,光纤探测口与反应孔对准要求大大降低。试验结果表明,该光纤检测系统的检出限为0.63μg/L,标准曲线相关系数为0.9977,RSD为1.03%~2.38%,样品加标回收率为92.62%~96.03%,具有更高线性度、精密度、重现性等。

In order to solve the alignment problem between the detection head of the large-size photoelectric detection device and the reaction hole,a small,in the traditional chemiluminescence enzyme immunoassay method.A multi-mode optical fiber combined with the optical fiber-type photon counting probe is used to detect the target signal,and an aflatoxin B1optical fiber detection system based on photoelectric detection technology is designed.The ZEMAX simulation and test results show that the optical fiber photon collection ratio reaches more than 95.83%and RSD≤0.21%in the reaction hole range compared with the reaction hole center position detection,and the alignment requirements of the optical fiber detection port and the reaction hole are greatly reduced.The experimental results showed that the detection limit of the fiber optic detection system was 0.63μg/L.The correlation coefficient of the standard curve was 0.9977.The RSD was 1.03%~2.38%,and the sample spiked recovery was 92.62%~96.03%with higher linearity,precision,and reproducibility.