摘要
The CRISPR-Cas9 system,serving as a powerful genome-editing technology,has revolutionized the life sciences.However,it exhibits off-target activities that may present severe problems in clinical applications.Although a great number of in silico models have been developed to predict CRISPR targeting efficiency and specificity,they are running into a bottleneck with the lack of a mechanistic understanding of the on-and off-target activities of Cas9.
作者
Qinchang Chen
Guohui Chuai
Chao Zhang
Qing Zhang
Qi Liu
陈钦畅;啜国晖;张超;张清;刘琦(Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine,Shanghai East Hospital,Bioinformatics Department,School of Life Sciences and Technology,Tongji University,Shanghai 200092,China;Shanghai Research Institute for Intelligent Autonomous Systems,Shanghai 201210,China;Department of Plastic and Reconstructive Surgery,Shanghai Institute of Precision Medicine,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Roche Pharma Research and Early Development,pRED Informatics,Roche Innovation Center Shanghai,Roche R&D Center(China)Ltd.,Shanghai 201203,China)
基金
supported by the National Key Research and Development Program of China(2021YFF1201200,2021YFF1200900)
the National Natural Science Foundation of China(31970638,61572361,62102286,and 62002265)
Roche pRED Informatics Advanced Analytics Postdoctoral Fellowship Program(aligned with the Roche pRED Postdoctoral Fellowship Program RPF-500)
Shanghai Natural Science Foundation Program(17ZR1449400)
Shanghai Artificial Intelligence Technology Standard Project(19DZ2200900)
Shanghai Shuguang Scholars Project
We Bank Scholars Project and Fundamental Research Funds for the Central Universities。
