小鼠原位肺癌模型构建及在放射治疗研究中的意义

Establishment of mouse model of lung cancer in situ and its significance in study of radiation therapy research

目的:构建小鼠原位肺癌模型,探讨其在放射治疗研究中的意义。方法:采用10只C57BL/6J小鼠,于左肺上叶接种鼠源性小鼠肺癌细胞,构建小鼠原位肺癌模型,观察小鼠接种肿瘤期间饮食、体重变化、病理学诊断及肺部磁共振成像(MRI)表现。结果:实验小鼠模型通过MRI及病理学验证,10只肺癌模型均构建成功,成功率为100%,成瘤时间为移植后7~14 d期间,21 d影像提示小鼠出现肺内、胸膜及淋巴结转移,在23 d统一处死后未发现肝脏及肾脏转移。结论:以鼠源性肺癌细胞构建的原位肺癌模型可重复,造模时间短,成功率高,肿瘤模型具有良好的稳定性和一致性;MRI验证可明显且直观体现肿瘤生长期,为后续开展放射治疗体内实验研究打下良好基础。

Objective:To establish mouse model of lung cancer in situ and to explore its significance in the study of radiation therapy research.Methods:Ten C57BL/6J mice were inoculated with mouse-derived lung cancer cells in the upper lobe of the left lung to establish a mouse model of lung cancer in situ.Diet,body weight changes,pathological diagnosis and lung magnetic resonance imaging(MRI)findings during tumor inoculation were observed.Results:The experimental mouse models were verified by MRI and pathology.All the 10 lung cancer models were successfully constructed with a success rate of 100%.The tumor formation time was 7 to 14 days after transplantation.On the 21st day,imaging suggested intrapulmonary,pleural,and lymph node metastasis in mice.On the 23st day,no liver and kidney metastasis was found after after sacrifice.Conclusion:The in situ lung cancer model constructed with mouse-derived lung cancer cells is reproducible,with short modeling time and high success rate.The tumor model has good stability and consistency.MRI verification can clearly and intuitively reflect the tumor growth stage.It lays a good foundation for the subsequent in vivo experimental research of radiation therapy.