达格列净对大鼠心肌缺血再灌注损伤保护的实验研究

Protective effect of dapagliflozin on myocardial ischemia-reperfusion injury in rats

目的:通过心肌缺血再灌注损伤(I/R)的不同时间点急性给药达格列净,探讨钠-葡萄糖共转运蛋白2(SGLT-2)抑制剂对心功能的影响。方法:对40只大鼠进行同等程度的心脏I/R刺激。将大鼠分为四个干预组:对照组、预处理组、缺血组、再灌注开始时组,快速给予SGLT-2抑制剂(达格列净,1 mg/kg)。监测各组大鼠心肌梗死面积大小、左室功能和心律失常情况。结果:缺血前给予达格列净可降低心律失常发生的频率、减小心肌梗死面积,使心肌I/R损伤后左室功能得到改善,从而达到最大程度的心脏保护作用。缺血期间给予达格列净也显示出心脏保护作用,但效果较缺血前给药降低。结论:心脏I/R损伤时急性给药达格列净可通过减少心肌梗死面积、改善左室功能和减少心律失常而发挥心脏保护作用。

Objective:To investigate the effect of sodium-glucose cotransporter 2(SGLT-2)inhibitor on cardiac function by acute administration of dapagliflozin at different time points during myocardial ischemia-reperfusion(I/R)injury.Methods:Forty rats were subjected to the same degree of cardiac I/R stimulation.The rats were divided into 4 intervention groups:control group,preconditioning group,ischemia group,and reperfusion initiation group,and were rapidly given 1 mg/kg of dapagliflozin.The size of myocardial infarction,left ventricular function and arrhythmia in each group were monitored.Results:The administration of dapagliflozin before ischemia could reduce the frequency of arrhythmia and the area of myocardial infarction,and improve the left ventricular function after myocardial I/R injury,thus achieving the greatest degree of cardiac protection.The administration of dapagliflozin during ischemia also showed a cardioprotective effect,but the effect was lower than that before ischemia.Conclusion:Acute administration of dapagliflozin in patients with myocardial I/R injury can play a cardioprotective role by reducing the size of myocardial infarction,improving left ventricular function and reducing arrhythmia.