马尾松MAPK级联途径应答信号物质基因的表达分析

Expressions and of Genes Response to Signal Substances in MAPK Cascade Pathway Genes in Pinus massoniana

MAPK(mitogen-actived protein kinase)在响应和调控生物与非生物胁迫、激素调节等方面发挥重要作用。马尾松MAPK级联途径7个基因被鉴定,并进行分子特性和外源信号物质处理表达模式分析。结果表明,马尾松与火炬松、北美云杉等针叶裸子植物的关系较近,不同类型基因具有该类基因特有的MAPK结构域以及典型催化氨基酸基序,D(I/L/V)K是马尾松MAPK基因共有的活性基序,PmMAP4K与MAPK级联途径的3个成员未直接互作,MAPK级联途径基因可能通过磷酸化调控各种激素生物合成来响应非生物胁迫。7个基因均响应了MeJA、GA、SA、ABA、Ca^(2+)处理,但模式不同。PmMAP4K基因对Ca^(2+)处理响应明显,其次是PmMAP2K基因,其它基因对单独Ca^(2+)处理响应不明显,4种激素和Ca^(2+)共同处理均提高了基因表达量,MeJA+Ca^(2+)处理MAPK基因表达量高于单独MeJA处理,而GA、SA、ABA单独处理基因的表达量高于其与Ca^(2+)一起处理。以上结果为马尾松MAPK基因与信号物质途径互作应答非生物胁迫提供参考。

MAPK(mitogen-actived protein kinase)cascade pathway plays an important role in response and regulation of biotic,abiotic stresses,and hormone signals et al.In this study,7 genes related to MAPK cascade pathway in Pinus massoniana were identified.We analyzed their physical properties,functional domains,phylogenetic tree,conserved phosphorylation motifs,and gene interactions.We also tested the expression patterns of these genes under hormone and calcium treatment.The results showed that:these genes had close evolutionary relationships with gymnosperms,such as Pinus taeda and Picea asperata.These 7 genes had MAPK domain and typical catalytic amino acid motif.D(I/L/V)K was the common active motif of MAPKs genes of Pinus massoniana.PmMAP4K did not interact directly with other three members of MAPK cascade pathway.Genes in MAPK cascade might regulate the biosynthesis of various hormones by phosphorylation in response to abiotic stress.The results of gene expression showed that all the seven genes responded to MeJA,GA,SA,ABA and Ca^(2+)treatments,but the expression patterns were different.The results showed that the response of PmMAP4K to Ca^(2+)treatment was the most obvious followed by PmMAP2K gene,and other genes had no obvious response to Ca^(2+)treatment only.Four hormones and hormone-plus-Ca^(2+)treatments increased the expression of genes,respectively.The expression of PmMAPK in MeJA+Ca^(2+)treatment was higher than that in MeJA treatment alone,while the expression of genes under treatments of each GA,SA,and ABA was higher than that under treatments of each GA,SA,ABA plus Ca^(2+).This study provides theoretical support for the analysis of the interaction between MAPK cascade pathway genes and signal pathway in respond to abiotic stress.