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MicroRNA-939在甲状腺癌进展中的作用及机制 被引量:1

The function and mechanism of microRNA-939 in the progression of thyroid cancer
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摘要 目的探讨microRNA-939(miR-939)在甲状腺癌中的作用及机制。方法qPCR分析30对甲状腺癌患者组织中miR-939和CCND3的表达水平。通过CCK-8实验、平板克隆形成实验和Transwell侵袭迁移实验研究miR-939在甲状腺癌中的生物学行为。通过TargetScan预测miR939的靶基因,通过双荧光素酶活性测定、Western blotting和qPCR进行验证。结果miR-939模拟物过表达miR-939显著抑制TPC-1细胞的增殖、迁移和侵袭,而CCND3沉默后可以逆转miR-939过表达引起的TPC-1细胞的生物学变化。结论miR-939/CCND3信号通路是甲状腺癌治疗的新靶点。 Objective To illustrate the function and mechanism of miroRNA-939 in thyroid cancer.Methods qPCR was used to analyze the expression levels of miR939 and CCND3 in 30 pairs of tissue from patients with thyroid cancer.The biological roles of miR-939 in thyroid cancer were performed via CCK-8 assay,colony formation assay and Transwell invasion and migration assays.Target genes of miR-939 were predicted by Target Scan and verified via a dual luciferase activity assay,western blotting and qPCR.Results The overexpression of miR-939 using miR-939 mimics markedly inhibited the proliferation and migration and invasion of TPC-1 cells.CCND3 silencing reverses the biological changes in TPC-1 cells induced by miR-939 overexpression.Conclusion The miR-939/CCND3 signaling pathway is a new target for thyroid cancer therapy.
作者 刘雅歆 隋亚那 赵建鑫 吴红玉 王文文 LIU Yaxin;SUI Yana;ZHAO Jianxin;WU Hongyu;WANG Wenwen(Department of Ultrasound,Weifang Hospital of Traditional Chinese Medicine,Weifang 261041,China;Department of Emergency,Weifang Hospital of Traditional Chinese Medicine;Department of Joint Orthopedics,Weifang Hospital of Traditional Chinese Medicine)
出处 《潍坊医学院学报》 2022年第2期85-88,共4页 Acta Academiae Medicinae Weifang
基金 潍坊市科技发展计划项目资助课题(项目编号:2019YX016)。
关键词 甲状腺肿瘤 microRNA-939 CCND3 侵袭和迁移 增殖 Thyroid cancer MicroRNA-939 CCND3 Migration and invasion Proliferation
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