过表达阿尔茨海默病细胞模型中lncRNA SNHG16后DNA甲基转移酶1的变化

Changes of DNA methyltransferase 1 after lncRNA SNHG16 overexpression in Alzheimer's disease cell model

目的:探讨过表达阿尔茨海默病(Alzheimer′s disease,AD)细胞模型中lncRNA SNHG16后DNA甲基转移酶1(DNMT1)的变化,为治疗阿尔茨海默病提供新的思路。方法:实验分为SH-SY5Y组;SH-SY5Y+Aβ1-42组;SH-SY5Y+Aβ1-42+pcDNA3.1(+)组;SH-SY5Y+Aβ1-42+pcDNA3.1(+)-SNHG16组进行,采用CCK 8检测法检测各实验组细胞的存活率;应用Real-time PCR实时荧光定量PCR检测各个实验组细胞中SNHG16和DNMT1的mRNA表达情况;用免疫细胞化学检测法检测各个实验组细胞中DNA甲基化转移酶1(DNMT1)蛋白的表达量。结果:通过比较SH-SY5Y组和SH-SY5Y+Aβ1-42组,后者SNHG16和DNMT1的mRNA表达降低;SH-SY5Y+Aβ1-42+pcDNA3.1(+)-SNHG16组较SH-SY5Y+Aβ1-42组DNMT1蛋白表达增加。结论:过表达lncRNA SNHG16可能提高阿尔茨海默病细胞模型中DNA甲基化转移酶1的表达量。

Objective:To investigate the changes of DNA methyltransferase 1(DNMT1)after lncRNA SNHG16 overexpression in Alzheimer's disease(AD)cell model,and to provide new ideas for the treatment of AD disease.Methods:SH-SY5Y group,SH-SY5Y+Aβ1-42 group,SH-SY5Y+Aβ1-42+pcDNA3.1(+)group,and SH-SY5Y+Aβ1-42+pcDNA3.1(+)-SNHG16 group was used to detect the cell viability by CCK8 assay.Real-time PCR was used to detect the mRNA expression of SNHG16 and DNMT1 in each experimental group.The expression of DNA methyltransferase 1(DNMT1)protein in each experimental group was detected by immunocytochemistry.Results:By comparison,the mRNA expression of SNHG16 and DNMT1 in SH-SY5Y+Aβ1-42 group was lower than that in SH-SY5Y group;the expression of DNMT1 protein in SH-SY5Y+Aβ1-42+pcDNA3.1(+)-SNHG16 group was higher than that in SH-SY5Y+Aβ1-42 group.Conclusion:Overexpression of lncRNASNHG16 may increase the expression of DNA methyltransferase 1 in Alzheimer's disease cell model.