摘要
【目的】探究宽谱大肠杆菌O157∶H7噬菌体的生物学特性和全基因组特征。【方法】采用双层琼脂平板法从广西南宁某猪场污水样品中分离宽谱大肠杆菌O157∶H7裂解性噬菌体,通过空斑试验、噬斑试验对噬菌体进行宿主谱和裂解效价的测定;利用透射电镜观察、最佳感染复数测定、一步生长曲线测定、热稳定性与酸碱稳定性评估、杀菌试验及全基因组测序的方法分析噬菌体的形态学、生物学和全基因组特征。【结果】试验成功分离1株宽谱大肠杆菌O157∶H7裂解性噬菌体,命名为vB_EcoM_GXBP08。噬菌体vB_EcoM_GXBP08能高效裂解14株大肠杆菌,裂解效价可达10^(9)~10^(10)PFU/mL。以大肠杆菌O157∶H7 CVCC4050为宿主菌测得噬菌体的最佳感染复数(multiplicity of infection,MOI)为1。一步生长曲线显示,该噬菌体的潜伏期和爆发期分别为20和50 min,爆发量为154 PFU/cell,耐受温度范围为30~70℃,在pH为4.0~10.0的环境中能维持其活性。在宿主菌CVCC4050中的杀菌试验结果显示,MOI为1时噬菌体vB_EcoM_GXBP08具有良好的杀菌效果。根据透射电镜观察和全基因组序列分析,该噬菌体属于有尾噬菌体目肌尾噬菌体科T4-like噬菌体属,基因组片段总长为108114 bp,GC含量为36.23%。噬菌体vB_EcoM_GXBP08基因组中含有8个CDS,编码与噬菌体裂解有关的蛋白,未发现与抗生素耐药性、毒力因子、毒素和溶源性相关的基因。【结论】噬菌体vB_EcoM_GXBP08宿主谱宽、效价高,且温度、酸碱稳定性良好,在液体培养基中的杀菌能力强,本研究结果为后续研制宽谱噬菌体制剂以及在食品业、养殖业防治大肠杆菌O157∶H7感染中的应用提供参考。
【Objective】This study was aimed to explore the biological characteristics and genome characteristics of broad-spectrum Escherichia coli O157∶H7 phage.【Method】The broad-spectrum Escherichia coli O157∶H7 lytic phage was seperated from a swine farm sewage in Nanning of Guangxi using the double-layer agar culture method.The host range and the corresponding titers of phage were determined through the methods of spot tests and plaque tests.The methods of transmission electron microscope observation,determination of the best multiplicity of infection in the plural,one-step growth curve drawing,thermal sensitivity and pH stability evaluation,sterilization experiment and the whole genome sequencing were used to analyze the morphological features,biological characteristics and the whole genomic characteristics of phage.【Result】A broad-spectrum lytic phage against Escherichia coli O157∶H7 was successfully isolated and purified which was named as vB_EcoM_GXBP08.Phage vB_EcoM_GXBP08 could lyse 14 Escherichia coli strains with high efficiency,and the phage titers could reach 10^(9) to 10^(10) PFU/mL.Using Escherichia coli O157∶H7 CVCC4050 as host bacteria,the optimal multiplicity of infection(MOI)of phage vB_EcoM_GXBP08 was 1.One-step growth curve indicated that the latent period of vB_EcoM_GXBP08 was 20 min,the outbreak period was 50 min,and the outbreak quantity was 154 PFU/cell.The tolerable temperature range of phage vB_EcoM_GXBP08 was 30 to 70℃,and it could maintain activity at pH 4.0 to pH 10.0.The results of bactericidal experiment against CVCC4050 showed that phage vB_EcoM_GXBP08 had good germicidal efficacy when MOI was 1.According to transmission electron microscope observation and the whole genome analysis,phage vB_EcoM_GXBP08 belonged to Caudovirales order,Myoviridae family,T4-like Phagus,which had a genome consisting of 108114 bp with a GC content of 36.23%.Phage vB_EcoM_GXBP08 genome harbored 8 CDS associated with lysis proteins and lacked virulence genes and drug resistance genes associated with antibiotic resistance,toxins and virulence factors.【Conclusion】Phage vB_EcoM_GXBP08 had a broad host range,high titer,good thermal stability and acid-base stability,and a strong bactericidal effect in liquid medium.The results provided reference for the development of broad-spectrum phage and its application in the prevention and treatment of Escherichia coli O157∶H7 infection in food industry and breeding industry.
作者
周雨晴
马东鑫
吴洁
王晓晔
ZHOU Yuqing;MA Dongxin;WU Jie;WANG Xiaoye(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Fangchenggang Administrative Examination and Approval Service Center,Fangchenggang 538000,China)
出处
《中国畜牧兽医》
CAS
北大核心
2022年第7期2735-2745,共11页
China Animal Husbandry & Veterinary Medicine
基金
广西自然科学基金项目(2021GXNSFAA196058)
福喜乐分院科技计划项目广西大学福喜乐分院专项(福喜乐20210014)
崇左市科技计划项目牛疫病防控关键技术研究与应用(崇科FA2019006)
巴马县人才科技计划项目广西大学巴马分院专项(巴人科20210015,巴人科20210008)
南宁市良庆区科学研究与技术开发计划(202109)
南宁市科学研究与技术开发计划(20212023)
南宁市武鸣区科学研究与技术开发计划(20190101,20210102)
防城港市科学研究与技术开发计划(防科AB21014016)。