QuEChERS-超高效液相色谱-质谱联用技术测定果蔬中5种农药残留的方法优化

Optimization of QuEChERS-Ultra Performance Liquid Chromatography-Mass Spectrometry for Determination of 5 Pesticide Residues in Fruits and Vegetables

本文基于超高效液相色谱-质谱联用技术建立了同时检测草莓和生姜中5种典型农药残留的快速检测方法。从盐析剂和净化材料两方面对QuEChERS方法进行了优化,并对液相色谱条件及质谱条件进行了优化。结果显示,所有组分在1~50 ng/mL线性良好,相关系数(R^(2))均大于0.99,检出限为0.0005~0.0030 mg/kg,定量限为0.0015~0.0090 mg/kg;3个加标浓度(0.1 mg/kg、0.02 mg/kg、0.004 mg/kg)下,5种农药组分的平均回收率为82.4%~116.3%,相对标准偏差为4.3%~8.3%。本方法具有前处理简单、快速、准确、高灵敏度和高选择性等特点,适用于果蔬农药残留的快速检测。

A rapid method for simultaneous determination of 5 typical pesticide residues in strawberry and ginger was developed based on ultra performance liquid chromatography-mass spectrometry.The QuEChERS method was optimized from 2 aspects of salting out agent and purifying material,and the conditions of liquid chromatography and mass spectrometry were optimized.All the components showed good linearity in the concentration range of 1 to 50 ng/mL,with correlation coefficients(R2)greater than 0.99 and limits of detection of 0.0005 mg/kg to 0.0030 mg/kg.Limits of quantification were 0.0015~0.0090 mg/kg,and the average recoveries were 82.4%~116.3%with relative standard deviations of 4.3%~8.3%at 3 spiked concentrations(0.1 mg/kg,0.02 mg/kg,0.004 mg/kg).This method has the advantages of simple,rapid,accurate,high sensitivity and high selectivity,and is suitable for rapid determination of pesticide residues in fruits and vegetables.