脱水猪粪衍生生物炭在猪废水厌氧消化处理中的高效潜能

High-efficiency Treatment Potential of Dewatered Swine Manure-derived Biochar in Anaerobic Digestion of Swine Wastewate

本文探索了脱水猪粪衍生生物炭(DSMB)作为添加剂用于养猪废水厌氧消化资源回收和处理效率提高的可能性。在20 g/L的DSMB的添加条件下,猪废水的产甲烷滞后期缩短了2~3 d,最大产甲烷能力提高了18.29%~23.58%。添加DSMB可显著加快猪废水中可溶性COD和VFA的降解速率,其中温度300℃下制备的DSMB在厌氧消化系统中的性能优于在500℃和700℃下制备的DSMB。微生物群落解析结果表明,300℃温度下制备的DSMB中Thermotogae的相对丰度是对照组的3倍多,加速了水解和酸化阶段的发生。IN T-ETS活性和EEC结果分析表明,300℃下制备的DSMB作为氧化还原活性介质,通过潜在的直接种间电子传递(DIET)途径加速甲烷的生成。

This work explored the feasibility of dewatered swine manure-derived biochar(DSMB)as an additive to facilitate anaerobic digestion(AD)of swine wastewater for energy recovery and antibiotic resistance genes(ARG)attenuation enhancements.With 20 g/L DSMB assistance,the methanogenic lag time of swine wastewater was shortened by 2~3 days,and the maximum methane production was increased by 18.29%~23.58%.The addition of DSMB could significantly accelerate the degradation rate of soluble COD and VFA,and the performance of DSMB prepared at 300℃is better than that prepared at 500℃and 700℃.The results of microbial community analysis show that the relative abundance of Thermotogae in DSMB prepared at 300℃is more then three times that in the control group,which accelerates the accurrence of hydrolysis and acidification stages.The IN T-ETS activity and EEC analysis show that DSMB prepared at 300℃acts as redox active medium to accelerate methane production through potential direct interspecies electron transfer(DIET)pathway.