绿豆蛋白多肽脱苦条件优化

Optimization of Detoxification Conditions for Mung Bean Protein Polypeptide

经初步纯化的多肽混合液进行浓缩后,采用β-环糊精包埋掩盖法进行脱苦条件优化,以β-环糊精的添加量、脱苦温度、脱苦时间为单因素进行试验,检测苦味值的变化,再经正交试验对脱苦条件进行优化。脱苦优化结果为β-环状糊精添加量2%,包埋温度40℃,包埋时间60 min,脱苦试验效果最佳。

After the concentration of the purified polypeptide mixture,β-cyclodextrin embedding and covering method was used to optimize the detoxification conditions.The addition amount ofβ-cyclodextrin,detoxification temperature and detox-ification time were used as single factors to test the change of bitterness value,and then the detoxification conditions were optimized by orthogonal experiment.The optimal detoxification results were as follows:β-cyclodextrin content was 2%,emb-edding temperature was constant at 40℃,embedding time was controlled at 60 min,and the optimal detoxification results were obtained.