出口梗阻型便秘病人的差异表达蛋白及其生物学功能-基于同位素标记相对和绝对定量的蛋白质组学研究

Differentially expressed proteins and their biological functions in patients with obstructed defecation syndrome——a proteomic study based on iTRAQ

目的分析出口梗阻型便秘(ODS)病人病变直肠组织的差异表达蛋白及其生物学功能。方法纳入ODS病人20例、混合痔脱垂病人(无便秘症状)10例,所有病人均行经肛门吻合器直肠切除术并收集手术切除直肠标本。利用同位素标记相对和绝对定量技术筛选ODS病人与混合痔脱垂病人之间的差异表达蛋白谱,并对所有的差异表达蛋白进行基因本体论(GO)分析、京都基因与基因组百科全书(KEGG)分析和信号肽(SignalP)分析。结果ODS病人与混合痔脱垂病人之间差异表达蛋白共164个(表达上调88个,表达下调76个),涉及的生物过程主要包括细胞过程、生物调节、代谢过程等,涉及的细胞组成主要包括生化过程、细胞结构等,涉及的分子功能主要包括结合位点、催化活性、分子功能调节等。涉及的信号通路主要包括神经退化疾病、内分泌与代谢疾病及免疫疾病等。结论ODS病人与非便秘病人直肠组织蛋白存在差异,差异表达蛋白所参与的生物学过程及涉及的信号通路可能参与ODS发病机制。

Objective To analysis the differentially expressed proteins and their biological functions in diseased rectal tissues of patients with obstructed defecation syndrome(ODS).Methods 20 patients with ODS and 10 patients with prolapse of mixed hemorrhoids(without constipation symptoms)were included.All patients underwent stapled transanal rectal resection(STARR)and then rectal specimens were collected.Isobaric tags for relative and absolute quantification(iTRAQ)was used to screen the differentially expressed protein profiles between patients with ODS and with mixed hemorrhoid prolapse,and all differentially expressed proteins were analyzed by Gene Ontology(GO)analysis,Kyoto Encyclopedia of genes and genomes(KEGG)analysis and signal peptide(signalP)analysis.Results A total of 164 proteins(88 up-regulated and 76 down-regulated)were differentially expressed between ODS patients and control part with mixed hemorrhoid prolapse.The biological processes involved mainly include cellular processes,biological regulation and metabolic processes.The cell components involved mainly include biochemical processes and cell structure.The molecular functions involved mainly include binding sites,catalytic activity and Molecular function regulation,etc.The signaling pathways involved mainly include neurodegenerative diseases,endocrine and metabolic diseases and immune diseases.Conclusion There are differences in rectal tissue proteins between ODS patients and non-constipation patients.The biological processes and signal pathways involved in the differentially expressed proteins may be connected with the pathogenesis of ODS.