谷子热激蛋白HSP90基因家族鉴定及分析

Identification and Analysis of Heat Shock Protein HSP90 Family Genes in Foxtail Millet

热激蛋白90(HSP90,heat shock protein 90)广泛参与生物体的生长发育,对研究信号转导、细胞周期调控、蛋白降解及转运等生理生化代谢具有重要研究价值。为全面解析谷子HSP90基因家族信息,找到重要耐旱SiHSP90的优异单倍型,基于谷子基因组数据,利用生物信息学的方法对谷子HSP90基因家族成员进行了鉴定分析。结果表明,从谷子基因组水平共鉴定到9个SiHSP90基因家族,分布于5条染色体上,内含子数1~19个不等,编码蛋白质氨基酸个数为404~818;系统进化树分析结果显示,谷子HSP90基因家族分为4组;亚细胞定位预测结果显示该基因家族主要位于细胞质和内质网;基于已发表的谷子转录组数据进行组织表达分析,不同HSP90基因家族在不同组织中的表达模式不同;顺式作用元件分析得到9个响应胁迫与激素调控元件,表明谷子HSP90基因家族可在不同非生物胁迫下进行转录调控,响应不同的环境胁迫。耐旱与旱敏感的表达谱分析表明,HSP90基因家族SiHSP90-9在两类材料的多个组织表现极显著差异表达,对532份谷子品种SiHSP90-9基因进行单倍型分析发现,基因区变异极少,启动子区变异丰富,共分为4个单倍型,结合表型分析发现H004是最优异耐旱单倍型,且核心变异位点位于结合茉莉酸甲酯的顺式作用元件核心序列。

The heat shock protein 90(HSP90)family members are widely involved in the growth and development of organisms as well as the transmission of stress signals.They are of significant importance in the study of signal transduction,cell cycle regulation,protein degradation and transport.In order to decipher the HSP90 gene family in foxtail millet(Setaria italica(L.)P.Beauv.),and identify their elite haplotypes being important in drought tolerance,the family members(referred SiHSP90s)were identified by taking use of the foxtail millet genome reference.Nine SiHSP90s members were identified on 5 chromosomes,with the intron number of 1 to 19,and the amino acids of deduced protein ranged from 404 to 818.Phylogenetic analysis suggested that SiHSP90s were divided into four groups.SiHSP90s were predicted to be localized in cytoplasm and endoplasmic reticulum.Based on the released foxtail millet transcriptome data,the transcriptional patterns of SiHSP90s in tissues were different.Nine stress and hormone regulatory elements were predicted via cisacting element analysis,indicating that the transcriptional reprogramming of SiHSP90s under different abiotic stresses might be present.Haplotype analysis of 532 foxtail millet varieties showed little variation in gene region but abundant variations in the promoter region,which enables defining four haplotypes.In conjugation with phenotypic analysis,it is found that the haplotype H004 is likely the most elite haplotype showing drought tolerant,possibly due to the core sequence variations of this haplotype at cis-acting element responding to the Methyl Jasmonate(MeJA)treatments.