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胆钙化醇饵粒高效液相色谱分析方法研究

Researchon the Determination of Cholecalciferol RB by HPLC
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摘要 本文采用高效液相色谱法,以甲醇:水=95:5为流动相,使用C_(18)色谱柱和二极管阵列检测器,在264nm波长下对试样中的胆钙化醇进行分离和定量分析。结果表明胆钙化醇的线性相关系数为0.9998;标准偏差为0.000748;变异系数为0.99%;平均回收率为101.04%。 The separation and quantitative analysis of Cholecalciferol RB were carried out by reverse phase HPLC,using methanol/water=95/5(V/V)as mobile phase,C_(18) column and DAD detector at 264nm wavelength.The results showed that the linear correlation coefficient of 0.9998,the standard deviation of 0.000748,the coefficient of variation of 0.99% and the average recovery of 101.04% was obtained.
作者 王玉萍 雷琪 付萍萍 马畅 Wang Yuping;Lei Qi;Fu Pingping;Ma Chang(Agricultural Inspection and Testing Center of Shanxi,Xi'an Shanxi 710003,China)
出处 《农药科学与管理》 CAS 2022年第6期19-23,30,共6页 Pesticide Science and Administration
基金 《农药产品中有效成分测定通用分析方法-液相色谱法》体系建设“除草醚等10个农药品种液相色谱分析方法建立”。
关键词 胆钙化醇 高效液相色谱 分离 分析 cholecalciferol HPLC separation analysis
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