摘要
Efficient red emissive carbon dots(CDs)in aqueous solutions are very scarce for high performance bioimaging applications.In this work,we report a one-step solvothermal treatment to synthesize pure red emissive CDs(FA-CDs)from citric acid and urea in formic acid without complicated purification procedures.Photoluminescence quantum yield(PLQY)of 43.4%was observed in their dimethyl sulfoxide solutions.High PLQY up to 21.9%in aqueous solutions was achieved in their bovine serum albumin(BSA)composites(FA-CDs@BSA)with significantly enhanced multiphoton fluorescence.The strong surface electron-withdrawing structure of FA-CDs caused by the high content of C=O groups contributes for their pure red emission.Owing to the significantly enhanced single and multi-photon red fluorescence and enlarged particle sizes after composing with BSA,in vivo tumor imaging and two-photon fluorescence imaging of blood vessels in mouse ear have been realized via intravenous injection of FA-CDs@BSA aqueous solutions.
基金
supported by the Natural Science Foundation of China(No.61922091)
the Science and Technology Development Fund of Macao SAR(0040/2019/A1,0073/2019/AMJ,0011/2019/AKP,0128/2020/A3,0131/2020/A3 and 0120/2020/A3)
Fund from the University of Macao(SRG2019-00163-IAPME)
Research and Development Grant for Chair Professor Fund from the University of Macao(CPG2020-00026-IAPME)
Research Grant(MYRG2019-00103-IAPME,MYRG2020-00164-IAPME)from the University of Macao.
